黄连素对TGF-β1诱导的肺癌A549细胞侵袭和迁移的影响

储菲; 姚扬; 李正红

doi:10.13898/j.cnki.issn.1000-2020.07.001

黄连素对TGF-β1诱导的肺癌A549细胞侵袭和迁移的影响

Effect of berberine on invasion and migration induced by transforming growth factor-β1 in lung cancer A549 cells

摘要

摘要:
目的观察黄连素对转化生长因子（TGF）-β1诱导的肺癌A549细胞侵袭、迁移的影响。
方法以肺腺癌A549细胞为研究对象，观察1.25、2.5、5、10 ng/mL TGF-β1分别在24、48 h对A549细胞形态的影响，采用划痕实验和Transwell法检测浓度下TGF-β1作用于A549细胞48 h后迁移和侵袭能力的变化，通过SRB法评价0~160 μmol/L黄连素体外对A549细胞毒活性以筛选出黄连素的安全浓度，观察安全浓度内的黄连素对TGF-β1诱导的肺癌A549细胞迁移和侵袭能力的影响。
结果在5 ng/mL TGF-β1刺激48 h后，A549细胞株表现出明显的上皮-间充质转化形态学变化和更强的侵袭、迁移能力。10 μmol/L浓度范围内的黄连素对A549细胞无细胞毒性。与TGF-β1组相比，2.5、5、10 μmol/L黄连素可以显著抑制A549细胞的侵袭，10 μmol/L黄连素可以显著抑制A549细胞的迁移。
结论黄连素对TGF-β1诱导的肺癌A549细胞的侵袭和迁移具有抑制作用。

Abstract:
ObjectiveTo observe the effect of berberine on the invasion and migration of lung cancer A549 cells induced by transforming growth factor (TGF)-β1.
MethodsThe effects of 1.25, 2.5, 5.0 and 10 ng/mL TGF-β1 on the morphology of A549 cells were observed at 24 and 48 h, respectively.Wound scratch assay and Transwell assay were applied to detect the changes of migration and invasion ability of A549 cells treated with different concentrations of TGF-β1 for 48 h.The cytotoxic activity of berberine at the concentration of 0-160 μmol/L in vitro was evaluated by SRB assay to find the safe concentration of berberine, then the effects of berberine at safe concentrations on the migration and invasion induced by TGF-β1 in A549 cells were determined.
ResultsA549 cells showed significant epithelial-mesenchymal transition morphological changes and stronger invasion and migration ability after 5 ng/mL TGF-β1 treatment for 48 h.Berberine within the concentration range of 10 μmol/L exhibited no cytotoxicity to A549 cells.Compared with TGF-β1 group, 2.5, 5, 10 μmol/L berberine significantly inhibited the invasion of A549 cells, and 10 μmol/L berberine obviously suppressed the migration of A549 cells.
ConclusionBerberine inhibits the invasion and migration of lung cancer A549 cells induced by TGF-β1.

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