Abstract: Objective: To investigate the distribution and drug resistance of major pathogens of nosocomial infection in intensive care unit(ICU).Methods: The pathogens data of 124 patients with nosocomial infection in ICU were retrospectively analyzed.Results: Among 178 samples,124 strains of Gram-negative(G^-) bacilli(79.8%),15 strains of Gram-positive(G⁺) bacilli(8.4%) and 21 strains of fungus(11.8%) were found.The most common Gram-negative bacilli,in turn,was followed by Pseudomonas aeruginosa,Klebsiella pneumoniae,Acinetobacter baumannii and Burkholderia cepacia.The most common Gram-positive bacteria,in turn,was Staphylococcus aureus,Staphylococcus haemolyticus and Enterococcus faecalis.The most common fungus,in turn,was Candida albicans and Candida glabrata.The resistance rates of Pseudomonas aeruginosa to cefoperazone/sulbactam,amikacin,tobramycin and polymyxin E were less than 30%.The drug resistance rate of Klebsiella pneumoniae was more than 40%,its resistance rates to amikacin,Ciprofloxacin,tobramycin and cotrimoxazole were less than 45%.The resistance rates of Acinetobacter baumannii to cefoperazone/sulbactam,imipenem and meropenem were less than 20%,its resistance rates to other antibiotics were more than 70%.The resistance of Staphylococcus aureus and Staphylococcus haemolyticus to vancomycin and teicoplanin was not found.The Candida albicans was high sensibility to common antifungal drug.Conclusions: The main pathogen of nosocomial infection in ICU is Gram-negative bacilli,and the bacteria resistance to antibiotics is common.Close monitoring the common pathogenic bacteria and its sensibility to antibiotics in ICU is important in guiding the rational use of medicines and improving the curative effects of anti-infection.