Abstract: Objective:To investigate the effects of linear alkylbenzenesulfonate(LAS) om the oxidative stress damage of mouse skin and antagonism of isoflavone.Methods:Forty healthy male Kunming mice were randomly divided into the control group,low-dosage LAS group(150 mg/L),middle-dosage LAS group(300 mg/L) and high-dosage LAS group(600 mg/L)(10 mice each group).The distilled water and different dosage LAS were smeared on the back skin of mice once a day for 60 days.Fifty mice were randomly divided into the control group,LAS oxidative stress damage model group(skin smeared with 300 mg/L of LAS) and three antagonistic groups(skin smeared with 300 mg/L LAS after smearing 50,100 and 150 mg/L of isoflavone,once a day for 60 days).The content of malondialdehyde(MDA)and activities of super oxidase dimutase(SOD) and glutathione peroxidase(GSH-Px) in all mice skin were detected.Results:The content of MDA in mice skin smeared with LAS was remarkably higher than that in control group(P<0.01),and the contents of MDA in high-dosage LAS group were significantly higher than that in low- and middle-dosage LAS groups(P<0.01).The activities of SOD and GSH-Px in middle- and high- dosage LAS group were obviously lower than those in control group(P<0.01),which decreased with the dosage increasing of LAS(P<0.01).After isoflavone intervention,the contents of MDA in all antagonistic groups were lower than that in LAS damage group,and higher than that in control group(P<0.05 to P<0.01).Except the antagonistic group 1,the differences of the GSH-Px activity between other antagonistic groups and LAS damage groups were not statistically significant(P>0.05),the activities of SOD and GSH-Px in antagonistic groups were higher than those in LAS damage groups,and lower than those in control group(P<0.05 to P<0.01).The antagonistic effects increased with the isoflavone concentration increasing.Conclusions:LAS can lead to the oxidative stree damage of mouse skin,and the isoflavone can restrain the damage within a certain range of dosage.