Abstract: Objective:To investigate the roles of the aberrant expressions of vascular endothelial growth factor(VEGF)-D and gene of phosphate and tension homology deleted on chromsome ten(PTEN) in breast cancer tissue and its clinical significance.Methods:The levels of VEGF-D and PTEN in 90 samples of breast invasive carcinoma tissue, and 20 samples of benign proliferative lesions were detected using immunohistochemical method, the results of which together with the clinical pathological parameters were analyzed.Results:The differences of the levels of VEGF-D and PTEN protein between breast cancer tissue and benign hyperplastic lesions tissue were statistically significant(P<0.01).The differences of the positive expression of VEGF-D protein in the patients with lymph nodes metastasis or without, and positive and negative expression of estrogen receptor were statistically significant(P=0.03 and P<0.05).The differences of the negative expression of VEGF-D protein in the patients with lymph nodes metastasis or without, and positive and negative expression of estrogen receptor were statistically significant(P<0.01).The level of VEGF-D protein was significantly negative correlation with PTEN in breast cancer tissue(P<0.01).Conclusions:The aberrant expressions of VEGF-D and PTEN are related to the invasion and metastasis of breast cancer.The expressions of VEGF-D and PTEN protein are correlative.