Abstract: Objective:To investigate the effects of different doses of perfluorooctanoic acid(PFOA) on the spleen lipid peroxidation damage of mice.Methods:Forty SPF ICR mice were divided into the control group, and low(1 mg /kg), medium(5 mg /kg) and high dose(25 mg /kg) PFOA groups(10 mice each group) using the simple random sampling method.All mice were administered by gavage for 14 days.The mice were weighed every day, and their behavior were observed.The spleen/body weight indexes were calculated after 14 days.The activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), sorbitol dehydrogenase(SDH), lactate dehydrogenase(LDH), and the levels of malondialdehyde(MDA), nitric oxide(NO) were analyzed.Results:The body weight of mice treated with PFOA grew slowly or lightened, especially in middle and high dose group(P<0.01).The spleen/body weight indexes of mice treated with PFOA were significantly lighter than that in control group(P<0.05).Compared with control group, the levels of MDA NO and LDH increased, the activities of SOD, GSH-Px and SDH in mice treated with PFOA decreased obviously, respectively(P<0.01).Conclusions:PFOA can inhibit the body weight growth of mice, and induce the spleen lipid peroxidation damage.