The experimental study of spleen oxidation damage induced by perfluorooctanoic acid in mice
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摘要: 目的:探讨不同剂量全氟辛酸(perfluorooctanoic acid, PFOA)对小鼠脾脏组织的脂质过氧化损伤效应。方法:40只SPF级ICR小鼠, 按单纯随机抽样方法分为正常对照组和低剂量组(1 mg/kg PFOA)、中剂量组(5 mg/kg PFOAS)、高剂量组(25 mg/kg PFOA)3个染毒组, 每组10只。染毒组各组每天1次灌胃相应剂量PFOA染毒, 灌胃溶液体积为0.1 ml/10 g,正常对照组给予等体积0.9%氯化钠注射液, 持续14 d, 每天称质量1次, 观察行为, 14 d后称质量处死, 计算脾脏系数, 检测脾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、山梨醇脱氢酶(SDH)、乳酸脱氢酶(LDH)、丙二醛(MDA) 及一氧化氮(NO)水平。结果:各染毒组小鼠出现不同程度的体质量增长缓慢甚至减轻, 其中中、高剂量组出现明显减轻(P<0.01);各染毒组脾脏系数均明显高于对照组(P<0.05);与对照组相比, 各剂量组的脾组织匀浆中MDA、NO及 LDH 含量明显增高, SOD、SDH及GSH-Px 活性均明显降低(P<0.01)。结论:PFOA能抑制小鼠体质量的增长, 对脾脏组织造成一定的脂质过氧化损伤。Abstract: Objective:To investigate the effects of different doses of perfluorooctanoic acid(PFOA) on the spleen lipid peroxidation damage of mice.Methods:Forty SPF ICR mice were divided into the control group, and low(1 mg /kg), medium(5 mg /kg) and high dose(25 mg /kg) PFOA groups(10 mice each group) using the simple random sampling method.All mice were administered by gavage for 14 days.The mice were weighed every day, and their behavior were observed.The spleen/body weight indexes were calculated after 14 days.The activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-Px), sorbitol dehydrogenase(SDH), lactate dehydrogenase(LDH), and the levels of malondialdehyde(MDA), nitric oxide(NO) were analyzed.Results:The body weight of mice treated with PFOA grew slowly or lightened, especially in middle and high dose group(P<0.01).The spleen/body weight indexes of mice treated with PFOA were significantly lighter than that in control group(P<0.05).Compared with control group, the levels of MDA NO and LDH increased, the activities of SOD, GSH-Px and SDH in mice treated with PFOA decreased obviously, respectively(P<0.01).Conclusions:PFOA can inhibit the body weight growth of mice, and induce the spleen lipid peroxidation damage.
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Keywords:
- perfluorooctanoic acid /
- spleen /
- oxidative damage /
- mouse
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