Abstract: Objective:To investigate the clinical application value of single utility hole video-assisted thoracoscopic operation in the treatment of spontaneous pneumothorax.Methods:Fifty-five patients with spontaneous pneumothorax were treated with single utility hole video-assisted thoracoscopic operation(observation group, 33 cases) and three utility holes video-assisted thoracoscopic operation(control group, 23 cases), respectively.The operation time, bleeding volume, operation cost, and postoperative extubation time and complications between two groups were compared.Results:The bleeding volume and operation cost of observation group were lower than the control group(P<0.05), the differences of the operation time, postoperative extubation time and complications between two groups were not statistically significant(P>0.05 to P=1.000).Conclusions:The single utility hole video-assisted thoracoscopic operation is single, and do not increase the operation time, which is consistent with the concept of minimally invasive, and worth promoting in minimally invasive thoracic surgery.