Abstract: Objective:To investigate the distribution of irregular antibodies.Methods:The serums irregular antibodies in 23 940 patients were screened using microcolumn gel antiglobulin test card from April to December 2014,the positive specimens were specifically identified and analyzed.Results:The positive antibodies in 121 cases were detected,the positive rate of which was 0.51%.The proportion of screening in blood type systems of Rh-hr,Kidd,MNSs,Duffy and Lewis were 89.26%,1.65%,6.61%,1.65% and 0.83%,respectively.The positive detection rate in female patients(0.71%) was significantly higher than that in male patients(0.31%)(PConclusions:The irregular antibody in blood transfusion patients must be screened,especially for female patient.The blood type systems should be considered in identifying the irregular antibody at first.