Abstract: Objective:To explore the apoptosis-inducing effects of the application of the resveratrol(RES)combined with paclitaxel(PTX)on lung cancer cell line PC9,and its proable mechanism.Methods:After the PC9 cells were co-cultured with the single and combination of different concentrations two drugs,the anti-proliferative effects of drugs were measured using MTT,the cell cycle distribution and apoptosis were detected by flow cytometry,and the protein levels of Caspase-3,Bcl-2 and Bax were detected by western blotting.Results:The effects of RES and PTX on the inhibiting the prolification of PC9 cells were the time- and concentrate-dependent manner.The inhibition rate of RES combined with paclitaxel was higher than that of single RES or PTX use(P<0.01).The single use of RES or PTX could induce the apoptosis and block G₀/G₁ phase in PC9 cells,and the effect of which was significantly enhanced when two drugs were combined(P<0.01).Western blot assay showed that the changes of Caspase-3,Bcl-2 and Bax after the joint use of RES and PTX were significantly more than that of the single drug use.Conclusions:Both RES and PTX can inhibit the proliferation,induce apoptosis,block G₀/G₁ phase and increase the Caspase-3 activity of human lung cancer PC9 cells.The combined use of RES and PTX has synergistic effect,which can significantly promote the cell apoptosis,and the mechanism may be related to the upregulation of Bcl-2 and downregulation of Bax.