Abstract: Objective:To investigate the value of semen routine,biochemical criterion and spermatogenic cells in the diagnosis of azoospermia.Methods:The seminal spermatogenic cells was detected using the modified papanicolaou staining.The patients were divided into the group A(32 cases with spermatogenic cells) and group B(38 cases without spermatogenic cells) according to the presence or absence of seminal spermatogenic cells,30 normal men were set as the control group.The semen volume and pH,seminal plasma fructose content,neutral α-glycosidase activity and testicular biopsy in three group were detected.Results:The differences of the semen volume and pH and neutral α-glycosidase activity between group A and control group were not statistically significant(P>0.05),the seminal plasma fructose content in group A was significantly higher than that in group B and control group(P<0.01),and the semen volume and pH,and neutral α- glucosidase activity in group B were significantly lower than those in group A(P<0.01).The coincidence rates of the combined detection of seminal spermatogenic cells,semen routine and biochemical criterion,and testicular biopsy in the dagnoisis of obstructive and non-obstructive azzospermia were 97.3%(36/37) and 97.1%(33/34),respectively.Conclusions:The joint detection of seminal spermatogenic cells,semen routine and biochemical criterion can be used as an important indicator in differentiating the obstructive and non-obstructive azzospermia,which has important significance in guiding clinical treatment.