荧光定量RT-PCR技术检测健康人外周血γδT细胞细胞受体分子Vδ1~8亚群分布

孙垚; 汪洪涛; 陈立; 徐志庆; 宋传旺; 李柏青

doi:10.13898/j.cnki.issn.1000-2200.2015.09.002

荧光定量RT-PCR技术检测健康人外周血γδT细胞细胞受体分子Vδ1~8亚群分布

The distribution of Vδ1-8 subsets of T cell receptor molecule in the γδT cell of the human peripheral blood

摘要

摘要: 目的:检测和分析健康成人外周血中γδT细胞的T细胞受体(TCR)分子Vδ1~8亚群分布情况。方法:采集16名健康成人外周血,荧光定量反转录聚合酶链式反应检测其γδT细胞TCR分子Vδ1~8亚群的基因相对表达量,计算各亚群所占百分比。结果:健康成人外周血γδT细胞TCR分子Vδ1~8亚群的比例分别为:Vδ1(7.19±0.33)%、Vδ2(54.27±7.9)%、Vδ3(20.23±0.03)%、Vδ4(3.80±0.02)%、Vδ5(2.08±0.02)%、Vδ6(0.81±0.25)%、Vδ7(7.21±0.02)%、Vδ8(4.41±0.04)%。Vδ2亚群均明显高于其他亚群(P<0.01)。结论:我国健康成年人外周血γδT细胞TCR分子Vδ1~8亚群分布由多到少依次为Vδ2 >Vδ3 >Vδ1 >Vδ8 >Vδ7 >Vδ4 >Vδ5 >Vδ6。

Abstract: Objective:To observe the distribution of Vδ1 to 8 subsets of the T cell receptor(TCR) molecule in the γδT cell of the human peripheral blood using real-time PCR.Methods:The mRNA expressions of the Vδ1 to 8 subsets of TCR molecule in the γδT cell in 16 healthy people peripheral blood were detected by real-time PCR,the respective percentage of whose were calculated.Results:The ratio of Vδ1,2,3,4,5,6,7 and 8 subsets were(7.19±0.33)%,(54.27±7.9)%,(20.23±0.03)%,(3.80±0.02)%,(2.08±0.02)%,(0.81±0.25)%,(7.21±0.02)% and(4.41±0.04)%,respectively.The ratio of Vδ2 was significantly higher than that in other subsets(P<0.05).Conclusions:The distribution sequence from most to least of Vδ1 to 8 subsets of the TCR molecule in the γδT cell of the healthy people peripheral blood is Vδ2 >Vδ3 >Vδ1 >δV8 >Vδ7 >Vδ4 >Vδ5 >Vδ6.

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