• 中国科技核心期刊
  • 中国科技论文统计源期刊
  • 安徽省优秀科技期刊
  • 中国高校优秀期刊

STAT4、STAT6在子痫前期胎盘组织中的表达及意义

吕艳关, 臧晓娟, 武荣, 范广来, 刘石, 魏红

吕艳关, 臧晓娟, 武荣, 范广来, 刘石, 魏红. STAT4、STAT6在子痫前期胎盘组织中的表达及意义[J]. 蚌埠医科大学学报, 2015, 40(12): 1628-1630,1634. DOI: 10.13898/j.cnki.issn.1000-2200.2015.12.004
引用本文: 吕艳关, 臧晓娟, 武荣, 范广来, 刘石, 魏红. STAT4、STAT6在子痫前期胎盘组织中的表达及意义[J]. 蚌埠医科大学学报, 2015, 40(12): 1628-1630,1634. DOI: 10.13898/j.cnki.issn.1000-2200.2015.12.004
LÜ Yan-guan, ZANG Xiao-juan, WU Rong, FAN Guang-lai, LIU Shi, WEI Hong. The expressions of STAT4 and STAT6 in placenta of preeclampsia patients and its significance[J]. Journal of Bengbu Medical University, 2015, 40(12): 1628-1630,1634. DOI: 10.13898/j.cnki.issn.1000-2200.2015.12.004
Citation: LÜ Yan-guan, ZANG Xiao-juan, WU Rong, FAN Guang-lai, LIU Shi, WEI Hong. The expressions of STAT4 and STAT6 in placenta of preeclampsia patients and its significance[J]. Journal of Bengbu Medical University, 2015, 40(12): 1628-1630,1634. DOI: 10.13898/j.cnki.issn.1000-2200.2015.12.004

STAT4、STAT6在子痫前期胎盘组织中的表达及意义

基金项目: 

江苏省淮安市科技局立项项目(HAS2012023)

详细信息
    作者简介:

    吕艳关(1981-),男,硕士,主管检验师.

  • 中图分类号: R714.244

The expressions of STAT4 and STAT6 in placenta of preeclampsia patients and its significance

  • 摘要: 目的:探讨子痫前期患者胎盘组织中信号转导和转录激活因子(STAT)4以及STAT6的表达及意义.方法:采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接(SABC)法和Western blot法检测30名正常妊娠晚期孕妇(对照组)和50例子痫前期患者(子痫前期组,其中轻度22例、重度28例)胎盘组织中STAT4、STAT6的蛋白定位和表达.结果:STAT4主要表达于绒毛滋养细胞的胞质,胞核多数无明显着色;轻、重度子痫前期组胎盘组织中STAT4的表达均明显高于对照组(P<0.01);STAT6主要表达于绒毛滋养细胞的胞质,胞核多数无明显着色;轻度、重度子痫前期组均显著低于对照组(P<0.01),且轻、重度子痫前期组STAT4和STAT6表达水平差异均有统计学意义(P<0.01).子痫前期组和正常妊娠组绒毛组织中STAT4与STAT6的表达水平均呈负相关关系(r=-0.509,r=-0.617;P<0.01).结论:STAT4、STAT6共同参与子痫前期的病理生理改变,并与病情严重程度有一定关系.
    Abstract: Objective:To investigate the signal transduction and expression of STAT4 and STAT6 in placenta of preeclampsia patients and its significance.Methods:The expressions and location of STAT4 and STAT6 in placenta of 30 normal late pregnant women(control group) and 50 preeclampsia patients(including 22 mild cases and 28 severe cases) were observed using Western blot and immunohistochemistry,respectively.Results:The expressions of STAT4 and STAT6 located mainly in the cytoplasm of villous trophoblast cell,the most nucleus were no clear coloring.The expressions of STAT4 and STAT6 were significantly higher and lower than those of control group,respectively(P<0.01).The differences of the expressions between STAT4 and STAT6 in placenta of mild and severe preeclampsia patients were statistically significant(P<0.01).The expressions of STAT4 in the cytoplasm of villous trophoblast cell in two groups were negative correlation with the level of STAT6(r=-0.509,r=-0.617;P<0.01).Conclusions:Both STAT4 and STAT6 paticipate the pathogenic process of preeclampsia,and are related to the severe degree of disease.The high expression of STAT4 and low expression of STAT6 in the villous trophoblast cell of preeclampsia patients can lead to Th1/Th2 imbalance,which may lead to the preeclampsia.
  • [1]

    Ilekis J,Reddy UM,Roberts JM.Preeclampsia--a pressing problem:an executive summary of a National Institute of Child Health and Human Development workshop[J].Reprod Sci,2007,14(6):508-523.

    [2]

    ACOG Committee on Practice Bulletins--Obstetrics.ACOG practice bulletin.Diagnosis and management of preeclampsia and eclampsia Number 33,January 2002[J].Obstet Gynecol,2002,99(1):159.

    [3]

    Olusi SO,Diejomaoh M,Omu A,et al.Interleukins in preeelampsia[J].Ann Saudi Med,2000,20(1):4-7.

    [4] 王越,叶路,付正英,等.妊娠高血压综合征发病中免疫调节机制的研究[J].免疫学杂志,2004,20(2):188-202.
    [5]

    Lund R,Ahlfors H.Identification of genes involved in the initiation of human Th1 or Th2 cell eommitment[J].J Immunol,2005,35(11):3307-3319.

    [6]

    Namjou B,Sestak AL,Armstrong DL,et al,High-density genotyping of STAT4 reveals multiple haplotypic associations with systemic lupus erythematosus in different raeial groups[J].Arthritis Rheum,2009,60(4):1085-1095.

    [7] 乐杰.妇产科学[M].7版.北京:人民卫生出版社,2008:137-138.
    [8]

    Sood R,Zehnder JL,Druzin ML,et al.Gene expression patterns inhuman placenta[J].Proc Natl Acad Sci U S A,2006,103(14):5478-5483.

    [9]

    Chaouat G,Zourbas S,Ostojic S,et al.A brief review of recent data on some cytokine expressions at the materno-foetal interface which might challenge the classical Th1/Th2 dichotomy[J].J Reprod Immunol,2002,53(1/2):241-256.

    [10]

    Chaouat G.Innately moving away from the Th1/Th2 paradigmin pregnancy[J].Clin Exp Immunol,2003,131(3):393-395.

    [11]

    Saito S.Cytokine network at the feto-maternal interface[J].J Reprod Immunol,2000,47(2):87-103.

    [12]

    Kaplan MH,Whitfield JR,Boros DL,et al.Th2 cells are required for the Sehistosoma mansoni egg-induced-ganulomatous response[J].Immunol,1998,160(4):1850-1856.

    [13]

    Tyler DR,Persky ME,Matthews LA,et al.Pre-assembly of STAT4 with the human IFN-α/β receptor-2 subunit is mediated by the stat4 N-domain[J].Mollmmunol,2007,44(8):1864-1867.

    [14]

    Kim SH,Gunst KV,Sarvetnick N.STAT4/6-dependent ifferential regulation of chenokinereceptots[J].Clin Immunol,2006,118(2/3):250.

    [15]

    Sancheez-Guajardo V Tanchot C,O'Malley JT,et al.Agonist-driven development of CD4+CD25+Foxp3+ regulatory Tcellsrequires a second signalmediated by stat6[J].J Immunol,2007,178(12):7550-7556.

    [16]

    Wurster AL,Tanaka T,Grusby MJ.The biology of Stat4 and Stat6[J].Oncogene,2000,19(21):2577-2584.

    [17]

    Deboy CA,Xin J,Byram SC,et al.Immune-mediated neuroprotection of axotomized Mouse facial motoneurons is dependent on the IL-4/STAT6 signaling pathway in CD4(+)T cells[J].Exp Neurol,2006,201(1):212-224.

    [18] 张冬丽,杨雪峰.原因不明复发性自然流产患者绒毛组织中STAT4和STAT6的表达[J].郑州大学学报:医学版,2010,1(45):42-44.
    [19]

    Yang Z,Chen M,Ellett JD,et al.Autoimmune diabete is bloeked Stat4-defieient nice[J].J Autoimmun,2004,223:191-200.

    [20]

    Tyler DR,Persky ME,Matthews LA,et al.Pre-assembly of STAT4 with the human IFN-α/β receptor-2 subunit is mediated by the stat4 N-domain[J].Mol Immunol,2007,44(8):1864-1867.

    [21]

    Wang Y,Evans JT,Rodrigulz F,et al.A tale of two STAT6 knock out mice in the induction of experiment autoimmunune encephalomyelitis[J].Neuroimmunol,2009,206(1/2):76-85.

  • 期刊类型引用(1)

    1. 唐秀凤, 高莹莹, 李晓曦, 陈宇恒, 于萍, 许利平, 刘仁慧. 淫羊藿苷和齐墩果酸协同地塞米松对糖皮质激素敏感型/抵抗型细胞内信号通路蛋白的影响. 医学研究生学报. 2018(09): 910-915 . 百度学术

    其他类型引用(1)

计量
  • 文章访问数:  3416
  • HTML全文浏览量:  427
  • PDF下载量:  326
  • 被引次数: 2
出版历程
  • 收稿日期:  2015-04-17

目录

    /

    返回文章
    返回