减毒结核分枝杆菌H37Ra感染小鼠模型的实验研究

    Study on the mouse model infected by Mycobacterium tuberculosis H37Ra

    • 摘要: 目的:研究减毒结核分枝杆菌(M.tb)H37Ra 感染小鼠模型及其病理损伤的机制。方法:将清洁级Balb/c小鼠分为对照组和M.tb模型组,各3只。将H37Ra按照每只小鼠1×106 CFU的剂量,经尾静脉注射感染小鼠,分别于感染7、14和21 d后,无菌操作取小鼠肺和脾脏组织,测量肺和脾脏的脏器系数,匀浆后行活菌培养;肺和脾脏组织冷冻切片,行HE染色观察病理损伤情况。结果:小鼠感染M.tb 14和21 d后,脾脏脏器系数均较感染7 d后显著升高(P<0.01和P<0.05)。感染7 d后,肺和脾脏组织匀浆均可检出活菌,脾脏组织H37Ra活菌量明显高于肺脏,并随感染时间逐渐增加;感染14 d后,肺组织病理观察可见明显感染性病变损伤。结论:M.tb H37Ra 经尾静脉注射感染小鼠模型可造成部分结核病样病理性损伤。

       

      Abstract: Objective: To Study on the pathological lesion in Balb/c mice infected with H37Ra. Methods: The mice were divided into control group and model group infected with Mycobacterium tuberculosis. Balb/c mice was injected via the tail vein with attenuated Mycobacterium tuberculosis H37Ra (1×106 CFU/mouse). After infection, living bacteria, organ weight index of lung or spleen, and histopathological lesion were detected after 7, 14, and 21 days of infection. Results: Compared with the group of control, the organ coefficient of spleen increased statistically after 14 days or 21 days of infection(P<0.01 and P<0.05);The number of living bacteria in spleen tissue of infected mice increased with the days of infection and was higher in spleen tissue than that in lung tissue. Tuberculosis-like lesions were observed in spleen and lung tissue of infected mice model. Conclusions: Attenuated Mycobacterium tuberculosis H37Ra could induced tuberculosis-like lesions in Balb/c mouse via the tail vein injection.

       

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