Abstract: Objective: To summarize the common pathogenic bacteria and drug resistance of neonatal sepsis. Methods: The blood culture and sensitivity results of 96 cases of neonatal sepsis were analyzed retrospectively. Results: Ninety-seven strains of pathogenic bacteria were detected from the 96 cases of blood specimen, which included 74 strains of Gram-positive bacteria, 19 strains of Gram-negative and 4 strains of fungus. Gram-positive bacteria were highly resistant to penicillin, but completely sensitive to vancomycin;Gram negative bacteria were highly resistant to cephalosporin but not so resistant to meropenem;no bacterial strains were resistant to amikacin. Conclusions: The main pathogens of neonatal sepsis are Gram-positive coccio which are highly resistant to penicillins and cephalosporins.