Abstract: Objective: To investigate the situation of demodex infection in students in Chuzhou, and analyze its reasons for providing the experimental evidence in preventing and treating the demodex infection. Methods: One hundred and sixty-two medical students and 186 non-medical students were investigated using the transparent adhesive tape overnight method and self-made questionnaire. The demodex was observed using optical microscopy. Results: The infection rate of demodex in medical students was significantly lower than that in non-medical students(P<0.01), and the awareness rate of demodex in medical students was significantly higher than that in non-medical students(P<0.01). Conclusions: Popularizing the demodex knowledge and improving the demodex awareness have great significance in preventing the demodex infection and demodicidosis.