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    徐志庆, 夏惠, 李柏青. 不同刺激剂对健康人和结核患者外周血T细胞产生γ干扰素和肿瘤坏死因子-α的影响[J]. 蚌埠医科大学学报, 2016, 41(3): 288-291. DOI: 10.13898/j.cnki.issn.1000-2200.2016.03.003
    引用本文: 徐志庆, 夏惠, 李柏青. 不同刺激剂对健康人和结核患者外周血T细胞产生γ干扰素和肿瘤坏死因子-α的影响[J]. 蚌埠医科大学学报, 2016, 41(3): 288-291. DOI: 10.13898/j.cnki.issn.1000-2200.2016.03.003
    shu
    XU Zhi-qing, XIA hui, LI Bai-qing. Comparision of the different stimulants on the peripheral blood T cells of healthy people and tuberculosis patients producing interferon-γ and tumor necrosis factor-α[J]. Journal of Bengbu Medical University, 2016, 41(3): 288-291. DOI: 10.13898/j.cnki.issn.1000-2200.2016.03.003
    Citation: XU Zhi-qing, XIA hui, LI Bai-qing. Comparision of the different stimulants on the peripheral blood T cells of healthy people and tuberculosis patients producing interferon-γ and tumor necrosis factor-α[J]. Journal of Bengbu Medical University, 2016, 41(3): 288-291. DOI: 10.13898/j.cnki.issn.1000-2200.2016.03.003
    shu

    不同刺激剂对健康人和结核患者外周血T细胞产生γ干扰素和肿瘤坏死因子-α的影响

    Comparision of the different stimulants on the peripheral blood T cells of healthy people and tuberculosis patients producing interferon-γ and tumor necrosis factor-α

      摘要
    • 摘要: 目的:检测健康人和结核患者外周血经不同刺激剂刺激后T细胞亚群产生γ干扰素(IFN-γ)和肿瘤坏死因子-α(TNF-α)的变化。方法:12名健康人和12例结核患者的外周血单个核细胞用佛波醇脂(PMA)+钙离子霉素(IM)、结核杆菌耐热抗原(Mtb-HAg)、植物血凝素(PHA)刺激,莫能霉素阻断,流式细胞仪检测产生IFN-γ和TNF-α的T细胞亚群比例。结果:PMA+IM刺激健康人外周血αβ T细胞产生IFN-γ的比例明显高于结核患者(P<0.01);PMA+IM、Mtb-HAg和PHA刺激健康人外周血γδ T细胞产生IFN-γ的比例高于结核患者(P<0.05~P<0.01)。PMA+IM和PHA刺激健康人外周血αβ T细胞产生TNF-α的比例均高于结核患者(P<0.05);PMA+IM和PHA刺激健康人外周血γδ T细胞产生TNF-α的比例均高于结核患者(P<0.05)。结论:经不同刺激剂刺激后,健康人γδ T细胞产生IFN-γ的比例高于结核患者γδ T细胞产生IFN-γ的比例。经PMA和PHA刺激后,健康人αβ T和γδ T细胞产生TNF-α的比例高于结核患者。

       

      Abstract: Objective:To detect the changes of the different stimulants on the peripheral blood T cell subsets of healthy people and tuberculosis(TB) patients producing interferon-γ(IFN-γ) and tumor necrosis factor-α(TNF-α).Methods:The proportion of T cell subsets producing INF-γ and TNF-α in 12 healthy people peripheral blood mononuclear cells and 12 TB patients peripheral blood stimulated with PMA combined with ionomycin, Mtb-Hag, PHA and blocked with monensin were detected using the flow cytometry.Results:The proportion of INF-γ produced by αβ T cells of peripheral blood stimulated with PMA in healthy people was significantly higher than that in TB patients(P<0.05).The proportions of INF-γ produced by γδ T cells of peripheral blood stimulated with PMA, Mtb-Hag and PHA in healthy people were higher than that in TB patients(P<0.05 to P<0.01).The proportions of TNF-α produced by αβ T cells of peripheral blood stimulated with PMA PHA in healthy people were higher than those in TB patients(P<0.05).The proportions of INF-α produced by γδ T cells of peripheral blood stimulated with PMA and PHA in healthy people were higher than that in TB patients(P<0.05).Conclusions:After stimulated with different stimulants, the proportion of INF-γ produced by γδ T cells in healthy people is higher than that in TB patients.After simulated with PMA and PHA, the proportion of TNF-α produced by αβ T cells and γδ T cells in healthy people is higher than that in TB patients.

       

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