Abstract:
Objective: To investigate the value of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS) in
S. typhimurium homology analysis.
Methods: Forty-four
S. typhimuium strains isolated from Guangzhou were typed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, which was compared with pulse-field gel electrophoresis(PFGE) to analyze the main patterns of mass spectrogram.
Results: The PFGE results showed that 44
S. typhimuium strains could be divided into 15 types. At 80% of the clustering similarity, 44
S. typhimuium strains could be divided into 8 MALDI-TOF MS types, which was accordance to the PFGE results. The MS1(52.3%) was the main type, which contained multiple PFGE patterns.
Conclusions: The classification results of MALDI-TOF MS show the relative concentration of kinship of
S. typhimuium isolated from Guangzhou, which is a simple, rapid, high throughput method of classification.