Abstract: Objective: To investigate the clinical value of the dynamic detection of vascular endothelial growth factor(VEGF) and brain specific isoenzyme of creatine kinase(CK-BB) in neonates with hypoxic ischemic encephalopathy(HIE).Methods: Eighty-four neonates with HIE(HIE group),which included 33 mild cases,27 moderate cases and 24 severe cases,were intravenously injected with citicoline and 1,6 fructose diphosphate.Fifty-two normal newborns were set as the control group.The levels of VEGF and CK-BB in two groups were detected after 24 h,72 h and 7 d of birth.Results: After 24 h and 72 h of birth,the levels of VEGF in severe HIE,moderate HIE and mild HIE were gradual decreasing,which was significantly higher than that in control group.After 7 d of birth,the differences of the levels of VEGF between two groups were not statistically significant(P > 0.05).After 24 h,72 h and 7 d of birth,the levels of CK-BB in severe HIE,moderate HIE and mild HIE were gradual decreasing,the levels of CK-BB in HIE group were significantly higher than that in control group after 24 h and 72 h of birth(P < 0.01).After 7 d of birth,the levels of CK-BB in moderate and severe HIE patients were significantly higher than that in control group(P < 0.01),and the difference of the level of CK-BB between the mild patients and control group was not statistically significant(P > 0.05).Conclusions: The levels of VEGF and CK-BB in neonates with HIE increase significantly,the dynamic detection of which can help to determine the degree of brain injury in neonates with HIE,and has positive value in the diagnosis,treatment and prognosis of HIE.