Abstract: Objective: To investigate the distribution and drug resistance of pathogens isolated from blood culture to provide the basis in clinical rational use of antibiotics.Methods: The data of the pathogens isolated from blood culture and results of drug sensitive test were retrospectively analyzed from July 2011 to June 2012.The blood was cultured using the BACT/ALERT 3D automatic blood culture instrument.The bacteria identification and drug sensitive test were implemented using ATB expression identification system.Results: Seventy-six strains of pathogenic bacteria were isolated from clinical blood culture samples,which included 41 strains of gram-positive bacteria(53.95%),31 strains of gram-negative bacteria(40.79%) and 4 strains of fungi(5.26%).The top five pathogens were the Coagulase-negative staphylococci,Escherichia coli,staphylococcus aureus,Klebsiella pneumonia and Pseudomonas aeruginosa by turn.The sensitive rate of Escherichia coli and Klebsiella penumonia to carbapenem drug was 100%,the gram-positive Cocci of vancomycin-resistant was not found.Conclusions: The timely monitoring of the pathogenic bacteria change in blood culture and trend of drug resistance are important in guiding the clinical drug use.