过氧化氢对人脐静脉内皮细胞白细胞介素-37表达的影响

刘倩; 周庆

doi:10.13898/j.cnki.issn.1000-2200.2016.11.004

过氧化氢对人脐静脉内皮细胞白细胞介素-37表达的影响

刘倩,
周庆

Effect of hydrogen peroxide on the expression of in terleukin-3 in hurnan umbilical vein endothelial cells

LIU Qian,
ZHOU Qing

摘要

摘要: 目的：研究过氧化氢人脐静脉内皮细胞（H₂O₂）对白细胞介素（IL）-37表达的影响。方法：用实时荧光定量PCR法和Western blot法检测经系列浓度H₂O₂作用不同时间的人脐静脉内皮细胞中IL-37 mRNA和蛋白水平的变化。结果：实时荧光定量PCR结果显示：不同浓度H₂O₂作用人脐静脉内皮细胞后，IL-37 mRNA水平升高，并呈浓度依赖（P<0.01）。Western blot结果表明，H₂O₂作用人脐静脉内皮细胞20 h后，IL-37蛋白水平较对照组显著升高（P<0.01）。结论：H₂O₂能够上调人脐静脉血管内皮细胞IL-37的表达，为进一步研究IL-37在细胞氧化应激状态下的作用奠定了一定基础。

Abstract: Objective: To investigate the effects of hydrogen peroxide(H₂O₂) on the expression of interleukin-37(IL-37) in EA.hy926 cells.Methods: The expressions of IL-37 mRNA and protein in different time-points of human umbilical vein endothelial cell treated with different concentration of H₂O₂ were detected by qRT-PCR and Western blot.Results: The results of qPCR showed that the levels of IL-37 mRNA in human umbilical vein endothelial cell treated with different concentration of H₂O₂ significantly increased,which was the concentration-dependent(P<0.01).The results of Western blot showed that the level of IL-37 protein in human umbilical vein endothelial cell treated with H₂O₂ for 20 h was significantly higher than that in control group(P<0.01).Conclusions: The expression of IL-37 in human umbilical vein endothelial cell can be up-regulated by H₂O₂,which may be helpful to study the role of IL-37 in cellular oxidative stress.

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