Abstract: Objective: To explore the relationship between the concentration of lipopolysaccharide binding protein(LBP) and premature rupture of membrane(PROM),and provide a reference in diagnosing PROM and subclinical intra-amniotic infection(IAI).Methods: Sixty PROM patients were divided into the premature PROM(pPROM) group and term PROM(tPROM) group(30 cases each group),and 30 normal full-term pregnancy women were set as the control group.The serum LBP levels before delivery in three groups were detected using ELISA.Fetal membrane samples after delivery were examined using pathology technique.Results: The concentrations of LBP in pPROM group,tPROM group and control group were(125.22±37.51)μg/mL,(102.87±20.90)μg/mL and(66.09±31.17)μg/mL,respectively,the differences of which were statistically significant(P<0.01).The LBP concentrations in pPROM group and tPROM group were significantly higher than that in control group(P<0.01).There was a positive correlation between the serum LBP concentration and inflammation degree of the chorionic membrane in pPROM group(P<0.01).The concentration of serum LBP increased gradually(P<0.01).Conclusions: The serum level of LBP has a certain value in diagnosing PROM and PROM complicated with subclinical intra-amniotic infection.