Abstract: Objective: To compare the clinical effects of 3D laparoscopy with traditional laparoscopy in partial nephrectomy,and evaluate their practicability and safety.Methods: Among 42 limited renal tumor patients scheduled by operation,which were treated with partial nephrectomy under 3D laparoscopy(3D group,22 cases) and traditional laparoscopy(traditional group,20 cases),respectively.All operations were completed by the same operators.The operation time,warm ischemia time,peroperative bleeding,operation cost and complications between two groups were compared.Results: The operation time and warm ischemia time in 3D group were significantly less than those in traditional group(P<0.01 and P<0.05).The differences of the operation cost and peroperative bleeding between two groups were not statistical significance(P>0.05).One incision with delayed healing in 3D group and traditional group were found,which cured after conservative treatment.The surgical margins in two groups were negative.All cases were followed up for 6 to 48 months,no recurrence and metastasis,and normal kidney function were identified in two groups.Conclusions: Compared with the traditional laparoscopy surgery,the vision and anatomic structure under 3D laparoscopy are clear.The 3D laparoscopy can help to shorten the operation and warm ischemia time,which is worthy of clinic application.