Abstract: Objective: To investigate the effects of the subjective well-being and organizational identification on the job performance in nurses of tertiary hospital.Methods: Two hundred and forty-two nurses from tertiary hospital were investigated using the self-compiled general information questionnaire,subjective well-being scale,organizational identification scale and job performance scale,the result of which was descriptively and relatively analyzed,and constructed the structural equation model.Results: The total scores of the subjective well-being scale,organizational identification scale and job performance scale were(75.27±10.49),(31.83±5.27) and(74.19±8.71),respectively.The dimension scores from high to down of the relationship performance,learning performance,task performance and innovation performance were(19.36±2.50),(18.48±2.65),(18.31±2.33) and(18.19±3.20),respectively.The subjective well-being was not related to the task performance(P>0.05).There were the positive relationship between subjective well-being,organizational identification and job performance(P<0.05).The structural equation model showed that the subjective well-being and organizational identification had a direct effect on job performance,and with organizational identification as an intermediary variable,the subjective well-being had a direct effect on job performance.The standardized path coefficients of the subjective well-being and organizational identification to the job performance were 0.30 and 0.33,respectively,and the standardized total effect of the subjective well-being on the performance was 0.39,and the exogenous variable of subjective well-being and organizational identification could explain 25% of the total variation of the internal variable job performance.Conclusions: The subjective well-being,job involvement and job performance in nurses of tertiary hospital is optimistic,but which still has the improvement room.The hospital and nursing administrators should improve the subjective well-being and organizational identification of nurse to increase job performance.