Abstract: Objective: To investigate the influencing factors of the slow healing of perineal wound after Miles operation of rectal cancer.Methods: The clinical data of 210 rectal cancer patients diagnosed by pathology were retrospectively analyzed after Miles operation.The influencing factors of the slow healing of perineal wound,which included the gender,age,preoperative nutritional status,intraoperative pelvic peritoneal repair or not,preoperative radiotherapy or not and preoperative and postoperative hyperbaric oxygen therapy,were investigated,and the correlation of the poor healing of incision with influencing factor were statistically analyzed.Results: The difference of the primary healing rate of incision in patients with different gender,age,preoperative nutritional status and peritoneal repair were not statistically significant(P>0.05).The difference of the primary healing rate of incision in patients with preoperative radiotherapy was significant lower than that in patients without preoperative radiotherapy(P<0.01),and the difference of the primary healing rate of incision in patients with preoperative and postoperative hyperbaric oxygen therapy was significant higher than that in patients without preoperative and postoperative hyperbaric oxygen therapy(P<0.05).Conclusions: After Miles operation of rectal cancer,the preoperative radiotherapy can delay the primary healing of incision,the preoperative and postoperative hyperbaric oxygen therapy can promote the primary healing of incision,and the hyperbaric oxygen therapy can be used to prevent the poor wound healing.