Abstract: Objective:To investigate the consumption of antibacterials and the drug resistance of the bacteria isolated from clinical samples for rational use of antibacterials.Methods:The defined daily dose(DDDs) of inpatients and the drug resistance of the bacteria isolated from clinical samples in 2012-2013 were analyzed.Results:In the 2 years from 2012 to 2013,the main clinical drug selection was cephalosporins,quinolones,cephamycins and other β-lactamase inhibitors.The drugs of great variation of DDDs included cefuroxime,ceftriaxone,cefmetazole,cefoxitin,pazufloxacin and fosfomycin.In 2013,the resistance rates of Escherichia coli,Pseudomonas aeruginosa all increased for cefuroxime,cefmetazole and fosfomycin(P <0.05 to P <0.01);the resistance rate of Acinetobacter baumannii all exceed 80% for cefmetazol and fosfomycin(P <0.05 and P <0.01),the resistance rate of Staphylococcus aureus increased significantly for levofloxacin(P<0.01).Conclusions:There was a positive correlation between the DDDs and bacterial resistance,with the increasing of DDDs,the bacterial resistance increased significantly;when DDDs fell,portion of bacterial resistance decreased.Active surveillance of bacterial resistance in hospital and effective measures to control the amount of antibacterials,guidance of clinical rational drug use should be carried out to reduce bacterial resistance.