Abstract: Objective: To observe the effects of the intraperitoneal cytokine-induced-killer(CIK) cell combined with immunomodulator in the treatment of malignant ascites. Methods: Fifty middle and advanced stage patients with liver,stomach and pancreas complicated with ascites were randomly divided into the DDP(cisplatin) group and CIK group(25 cases each group).The DDP group were treated with intraperitoneal DPP chemotherapy,and the CIK group were treated with CIK cell therapy combined with mannatide immunity regulating,twice a week for 8 times.The efficacy,adverse reaction and Karnofsky Performance Status(KPS) score between two groups were compared. Results: The total effective rate in CIK group(76.0%) was significantly higher than that in DDP group(40.0%)(P<0.01).The gastrointestinal disorder and bone marrow suppression were the main adverse reactions in DDP group.Except a case with transient body temperature increasing,no adverse reaction was found in CIK group.The improvement of white blood cells,albumin,appetite and KPS score in CIK group were significantly better than those in DDP group(P<0.01). Conclusions: The intraperitoneal CIK cell therapy can control the malignant ascites and improve the symptom of patients,which is little adverse reaction,and worthy of promotion in clinic.