高级检索
    郑丽坤, 张磊, 付岭, 刘金梅, 陈乃耀, 卢士红. 中性粒细胞上清液对人脐带间充质干细胞迁移的影响[J]. 蚌埠医科大学学报, 2017, 42(7): 845-847,851. DOI: 10.13898/j.cnki.issn.1000-2200.2017.07.002
    引用本文: 郑丽坤, 张磊, 付岭, 刘金梅, 陈乃耀, 卢士红. 中性粒细胞上清液对人脐带间充质干细胞迁移的影响[J]. 蚌埠医科大学学报, 2017, 42(7): 845-847,851. DOI: 10.13898/j.cnki.issn.1000-2200.2017.07.002
    shu
    ZHENG Li-kun, ZHANG Lei, FU Ling, LIU Jin-mei, CHEN Nai-yao, LOU Shi-hong. Effect of the neutrophil supernatant on the migration of human umbilical cord mesenchymal stem cells[J]. Journal of Bengbu Medical University, 2017, 42(7): 845-847,851. DOI: 10.13898/j.cnki.issn.1000-2200.2017.07.002
    Citation: ZHENG Li-kun, ZHANG Lei, FU Ling, LIU Jin-mei, CHEN Nai-yao, LOU Shi-hong. Effect of the neutrophil supernatant on the migration of human umbilical cord mesenchymal stem cells[J]. Journal of Bengbu Medical University, 2017, 42(7): 845-847,851. DOI: 10.13898/j.cnki.issn.1000-2200.2017.07.002
    shu

    中性粒细胞上清液对人脐带间充质干细胞迁移的影响

    Effect of the neutrophil supernatant on the migration of human umbilical cord mesenchymal stem cells

      摘要
    • 摘要: 目的：探讨中性粒细胞上清液和人脐带间充质干细胞（mesenchymal stem cells，MSCs）共培养后，对MSCs迁移的影响，为MSCs更好地应用于炎症性疾病的治疗提供理论依据。方法：分离脐血中性粒细胞，UC-MSCs和中性粒细胞按不同比例共培养后，提取中性粒细胞上清液，UC-MSCs单独培养的为对照组（1∶0），UC-MSCs在不同浓度的中性粒细胞上清液中进行培养的为实验组，选择18 h和40 h作为培养时间，根据四甲基偶氮唑盐（MTT）的实验结果，选择具有统计学意义的1∶0、1∶1、1∶50分组和18 h作为实验对象，采用划痕的方法观察24 h内不同时间点MSCs的迁移情况。结果：划痕实验观察到1︰50实验组细胞的迁移较1∶0和1∶1实验组明显增快。结论：高浓度的中性粒细胞的上清液促进人脐带MSCs的迁移。

       

      Abstract: Objective:To investigate the effects of neutrophil supernatant on the migration of human umbilical cord mesenchymal stem cells(UC-MSCs) for providing a theoretical basis in treating the inflammatory disease with MSCs.Methods:The neutrophil cells were isolated form umbilical cord blood,co-cultured with UC-MSCs,and the neutrophil supernatant was extracted.The only UC-MSCs and co-culturing of UC-MSCs with different concentrations of neutrophil supernatant were divided into the control group and experimental group,respectively.The 18h and 40h was set as the culturing time.According to the experimental results of methyl thiazolyl tetrazolium(MTT),the ratios of UC-MSCs to neutrophil supernatant for 1∶0,1∶1 and 1∶50 were investigated.The migration of MSCs at different time-points within 24h were observed using the scratch method.Results:The result of scratch showed that the migration speed of MSCs in 1∶50 cells was significantly quicker than that in 1∶0 and 1∶1 cells.Conclusions:The high concentration of neutrophil supernatant can promote the migration of human umbilical cord MSCs.

       

      • HTML全文
      • 参考文献(16)
      • 相关文章
      • 施引文献
    • 资源附件(0)

    /

    返回文章
    返回