长链非编码RNA MALAT1对胰腺癌细胞系PANC-1转移的影响

赵义; 王启之; 燕善军; 刘晓阳; 薛永举; 王猛; 崔艳艳

doi:10.13898/j.cnki.issn.1000-2200.2018.03.001

长链非编码RNA MALAT1对胰腺癌细胞系PANC-1转移的影响

Effect of long non-coding RNA MALAT1 on the PANC-1 metastasis of pancreatic cancer cell line

摘要

摘要: 目的：探讨长链非编码RNA肺腺癌转移相关转录本1（MALAT1）在胰腺癌细胞系中的表达及其对PANC-1转移的影响。方法：应用荧光定量PCR检测4种胰腺癌细胞系中MALAT1的表达水平，通过siRNA下调PANC-1的MALAT1水平，应用贴壁、离壁实验和Transwell迁移、侵袭实验观察PANC-1的贴壁、离壁、迁移和侵袭能力，应用Western blotting检测PANC-1的Vimentin、MMP-2和MMP-9蛋白表达水平。结果：4种胰腺癌细胞系中MALAT1差异性表达，PANC-1细胞系中表达最高。下调MALAT1的表达后，PANC-1的Vimentin、MMP-2和MMP-9的蛋白水平显著下降（P<0.01），贴壁、离壁、迁移、侵袭能力均显著减弱（P<0.01）。结论：下调MALAT1通过降低Vimentin、MMP-2和MMP-9的表达可减弱胰腺癌细胞系PANC-1的转移能力。

Abstract: Objective:To explore the expression of metastasis-associated lung adenocarcinoma transcript 1(MALAT1) long non-coding RNA in pancreatic cancer cells,and its effect on the metastasis of PANC-1.Methods:The expression levels of MALAT1 in 4 pancreatic cancer cell lines were analyzed by real-time PCR.The level of MALAT1 in PANC-1 was down-regulated by small interfering RNA.The abilities of attachment detachment,migration and invasion of PANC-1 were measured using the attachment assay,detachment assay,Transwell migration and invasion assay,respectively.The protein levels of Vimentin,MMP-2 and MMP-9 in PANC-1 were detected by Western blotting.Results:The expression levels of MALAT1 in 4 pancreatic cancer cell lines were different,and which was the highest in PANC-1.After down-regulating the level of MALAT1 in PANC-1,the protein levels of Vimentin,MMP-2 and MMP-9 significantly decreased(P<0.01),and the abilities of attachment detachment,migration and invasion of PANC-1 significantly weakened(P<0.01).Conclusions:Down-regulating the expression of MALAT1 can decrease the levels of Vimentin,MMP-2 and MMP-9,which can attenuate the capacity of PANC-1 metastasis.

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