Abstract: Objective:To investigate human papillomavirus(HPV) infection in cervix uteri among 116 441 census women in Minhang District of Shanghai,analyze HPV genotype,characteristic of age distribution and distribution characteristic of HPV genotype in women with cervical lesion.Methods:HPV genotyping was performed by high throughput sequencing technology to test 14 high-risk types of HPV and 2 low-risk types of HPV.Results:A total number of 115 463 cases were successfully detected,the infection rate of HPV and high-risk HPV was 11.18% and 10.93%,respectively.The highest infection rate was 15.90% founded in ≤ 25 years old group.Multiple infections were mainly found in >60 years old women.The top five of infection rate in general population was HPV52,HPV58,HPV16,HPV68 and HPV18.The rate of multiple infections was 21.02%,there were six kinds of infections at most.There were 208 cases of cervical cancer and precancerous lesion,in which 97.60% with HPV infection.All of the cervical cancer and CINⅢ cases were infected by high-risk HPV,the top five of infection rate was HPV16,HPV52,HPV58,HPV18 and HPV31.HPV16 was the first one and the rate of infection improved with the increasing grade of cervical lesion.Conclusions:Continued infection by HPV16,HPV52,HPV58 and HPV18 is the leading cause of cervical precancerous lesion and cervical cancer in women in Minhang District.HPV16 has the strongest carcinogenicity.After gynecological census,close follow-up and management of HPV16,HPV52,HPV58 and HPV18 infection are needed,it is especially necessary to pay attention to elderly women with HPV16 multiple infections.