基质金属蛋白酶-9、Ⅲ型胶原、纤维黏连蛋白在胎膜早破发病机制中的作用

田玲; 万敏; 王倩; 柏妍槟; 沈禾; 闫华

doi:10.13898/j.cnki.issn.1000-2200.2018.07.018

基质金属蛋白酶-9、Ⅲ型胶原、纤维黏连蛋白在胎膜早破发病机制中的作用

Role of matrix metalloproteinases-9,collagen Ⅲ and fibronectin in premature rupture of fetal membranes

摘要

摘要: 目的：观察并检测基质金属蛋白酶-9（MMP-9）、Ⅲ型胶原、纤维黏连蛋白（FN）在胎膜早破孕妇血清及胎膜组织中的表达水平，探讨其参与胎膜早破的发病机制。方法：40例胎膜早破孕妇，其中未足月胎膜早破组（pPROM组，20例），足月胎膜早破组（tPROM组，20例），另外选取同期正常足月妊娠孕妇20名作为对照组。应用酶联免疫吸附试验及免疫组织化学方法检测血清及胎膜组织中MMP-9、Ⅲ型胶原、FN的表达水平，并对测定结果进行相关性分析。结果：3组孕妇血清MMP-9、Ⅲ型胶原、FN含量差异均有统计学意义（P < 0.01），其中，tPROM组MMP-9显著高于pPROM组，后者又显著高于对照组（P < 0.01）；pPROM组Ⅲ型胶原、FN水平显著低于tPROM组，而后者又显著低于对照组（P < 0.01）。MMP-9在pPROM组和tPROM组胎膜组织中的阳性表达率均高于对照组（P<0.05）。MMP-9在tPROM组胎膜组织中的阳性表达率高于pPROM组，但差异无统计学意义（P > 0.05）。Ⅲ型胶原和FN在pPROM组和tPROM组胎膜组织中的阳性表达率均低于对照组（P<0.05）。Ⅲ型胶原和FN在tPROM组胎膜组织中的阳性表达率低于pPROM组，但差异无统计学意义（P > 0.05）。在pPROM组和tPROM组中，MMP-9和Ⅲ型胶原的表达水平均呈负相关关系（r=-0.874，r=-0.988，P<0.01），MMP-9与FN的表达水平均呈负相关关系（r=-0.760，r=-0.875，P<0.01），Ⅲ型胶原与FN的表达水平均呈正相关关系（r=0.529，r=0.876，P<0.05）。在胎膜组织中，MMP-9和Ⅲ型胶原的表达水平呈负相关关系（r=-0.468，P<0.01），MMP-9和FN的表达水平呈负相关关系（r=-0.493，P<0.01），Ⅲ型胶原和FN的表达水平呈正相关关系（r=0.885，P<0.01）。结论：MMP-9、Ⅲ型胶原、FN参与胎膜早破的发生，孕妇血清及胎膜组织中MMP-9含量升高、Ⅲ型胶原和FN含量降低，这些因素均可能导致胎膜早破的发生。

Abstract: Objective: To observe the expression levels of matrix metalloproteinases-9(MMP-9),type Ⅲ collagen and fibronectin(FN) in the serum and fetal membranes in women with premature rupture of membrane,and investigate their roles in premature rupture of membranes.Methods: Twenty women with preterm premature rupture of membranes,20 women with term fetal rupture of membranes and 20 women with normal full-term pregnancy were divided into the pPROM group,tPROM group and control group,respectively.The levels of MMP-9,type Ⅲ collagen and FN in the serum and fetal membranes in three groups were detected using the enzyme-linked immunosorbent assay and immunohistochemistry,and the results of those were analyzed.Results: The differences of the serum levels of MMP-9,type Ⅲ collagen and FN between three groups were statistically significant(P<0.01),the serum level of MMP-9 in tPROM group was significantly higher than that in pPROM group,and the serum level of MMP-9 in pPROM group was significantly higher than that in control group(P<0.01).The positive rates of MMP-9 in fetal membranes of pPROM group and tPROM group were significantly higher than that in control group(P<0.01),the positive rate of MMP-9 in tPROM group was significantly higher than that in pPROM group,and the difference of which between two groups was not statistically significant(P>0.05).The positive rates of type Ⅲ collagen and FN in fetal membranes of pPROM group and tPROM group were lower than that in control group(P<0.01),the positive rates of type Ⅲ collagen and FN in tPROM group was lower than that in pPROM group,and the difference of which between two group was not statistically significant(P>0.05).Among pPROM group and tPROM group,the level of MMP-9 was negatively related to the type Ⅲ collagen level(r=-0.874,r=-0.988,P<0.01),the level of MMP-9 was positively related to the FN level(r=-0.760,r=-0.875,P<0.01),and the level of type Ⅲ collagen was positively related to the FN level(r=0.529,r=0.876,P<0.05).In the fetal membrane tissue,the expression level of MMP-9 was negatively related to the level of type Ⅲ collagen(r=-0.468,P<0.01),the expression level of MMP-9 was negatively related to the level of type Ⅲ collagen(r=-0.493,P<0.01),and the level of type Ⅲ collagen was positively related to the level of FN(r=0.885,P<0.01).Conclusions: MMP-9,type Ⅲ collagen and FN are involved in the development of premature rupture of membranes.The levels of MMP-9 in serum and fetal membranes increasing and type Ⅲ collagen level decreasing can lead to the occurrence of premature rupture of fetal membranes.

HTML全文

参考文献(15)

施引文献

资源附件(0)