Abstract: Objective: To observe the clinical efficacy of Nd:YAG laser in the treatment of dentine hypersensitivity.Methods: A total of 200 patients with dentine hypersensitivity(380 ill teeth) were randomly divided into the control group and observation group(100 cases each group).The observation group(200 ill teeth) were treated with Nd:YAG laser desensitization,and the control group(180 ill teeth) were treated with Gluma desensitizer.The clinical efficacy between two groups after 0 hour,1 week,1 month,3 months,6 month and 12 month of treatment were compared.Results: The differences of the effective rates between two groups after 0 hour,1 week,1 month and 3 months of treatment were not statistically significant(P>0.05),and the differences of the effective rates between two groups after 6 and 12 months of treatment were significant(P<0.05).Conclusions: The Nd:YAG laser in the treatment of dentine hypersensitivity has good middle and long-term clinical effects.