Abstract: Objective: To evaluate the effects of open experiment teaching on cultivating innovation ability of undergraduates in Preventive Medicine speciality.Methods: Sixty undergraduates from grade 2016 Preventive Medicine speciality were divided into the observation group and control group(30 students each group).The observation group was taught with traditional experiment teaching combined with open experiment teaching,and the control group was taught with traditional experiment teaching.The innovation abilities between two groups were comprehensively evaluated in 2017.Results: The evaluation results showed that the environmental health,nutrition and food health,health care big data,and aging population between two groups were statistically significant(P<0.05 to P<0.01).The teaching effects on the innovation awareness and ability,critical thinking,teamwork spirit,wide vision and literature research ability in observation group were better than those in control group(P<0.05 to P<0.01).Compared with the control group,more students in observation group thought that the experimental course was very helpful for professional study,and future work and development(P<0.05 and P<0.01).Conclusions: The open experiment teaching reform is an important way to improve the experiment teaching effect in Preventive Medicine speciality undergraduates,which can improve the subjective initiative,creativity and professional quality.