Abstract: Objective: To establish the mouse model of hepatocellular carcinoma using rapid and vast tail vein injection.Methods: The mixed solution(2 mL) of transposon plasmid NRASV12 inserted by NRAS gene,transposon plasmid expressing activated AKT(myr-Akt) and transposase SB100 was injected into the tail vein of mice within 7 s(observation group).Mouse injected with the same volume saline was set as the control group.After 3 to 4 weeks of injection,the mice of the two groups were sacrificed,and the tumor formation was examined by pathology.Results: The tumor formation rate in observation group was 100%(24/24),and the hepatocellular carcinoma was identified by pathology.No tumor formation in control group was found.Conclusions: Establishment of the mouse model of hepatocellular carcinoma using rapid and vast tail vein injection is simple,has short cancer-inducing time,high success rate and good repeatability.