山莨菪碱预处理对大鼠肝缺血再灌注诱发肺损伤的影响

薛小红; 李璐; 周荣胜

doi:10.13898/j.cnki.issn.1000-2200.2019.01.004

山莨菪碱预处理对大鼠肝缺血再灌注诱发肺损伤的影响

Effect of anisodamine pretreatment on the lung injury induced by hepatic ischemia-reperfusion in rats

摘要

摘要:
目的探讨山莨菪碱预处理对大鼠肝缺血再灌注诱发肺损伤的影响。
方法48只SD健康雄性大鼠，采用随机数字表法，分为假手术组(S组)、肝缺血再灌注组(IR组)和山莨菪碱预处理组(A组)，各16只。S组只做解剖肝门，IR组和A组制备70%大部分肝缺血再灌注损伤模型。A组于阻断肝血流前30 min，静脉注射山莨菪碱2 mg/kg，S组和IR组静脉注射等剂量的0.9%氯化钠溶液。大鼠于再灌注后3 h处死，取肺脏组织，HE染色，光镜下观察病理学结果。计算肺湿/干重(W/D)比值。采用硫代巴比妥酸法测定丙二醛(MDA)，黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)，氧化氢还原法测定髓过氧化物酶(MPO)的表达；采用免疫组织化学法测定肺组织血红素氧合酶-1(HO-1)和诱导型一氧化氮合酶(iNOS)蛋白的表达。
结果肺组织HE染色病理改变，IR组和A组肺损伤较S组重，A组肺损伤较IR组轻；与S组比较，IR组和A组肺组织W/D比值、MDA含量、MPO活性、HO-1和iNOS蛋白表达均明显增高(P < 0.01)，SOD活性明显降低(P < 0.01)；与IR组比较，A组肺组织W/D比值、MDA含量、MPO活性、iNOS蛋白表达均明显降低(P < 0.01)，肺组织HO-1蛋白表达和SOD活性均明显升高(P < 0.01)。
结论山莨菪碱预处理可减轻大鼠肝缺血再灌注诱发的肺损伤。

Abstract:
Objective To investigate the effects of anisodamine pretreatment on the lung injury induced by hepatic ischemia-reperfusion in rats.
Methods Forty-eight healthy male Sprague-Dawley rats were randomly divided into the sham operation group(group S), hepatic ischemia-reperfusion group(group IR) and anisodamine group(group A) using a random number table(16 rats each group).The hepatic portal in group S was dissected, and the ischemia-reperfusion model of 70% liver in group IR and group A were established.The group A was injected with 2 mg/kg anisodamine before 30 min of blocking liver blood flow and the group S and group IR were injected with the same volume of 0.9% sodium chloride solution.The rats were sacrificed after 3 h of reperfusion, the lung tissue was harvested, stained using HE, and the result of which was observed under light microscope.The ratio of wet to dry(W/D) of lung weight was calculated.The levels of malondialdehyde(MDA), superoxide dismutase(SOD) and myeloperoxidase(MPO) in lung tissue were detected using thiobaituricacid, xanthinoxidase and Hydrogen oxide reduction method, respectively.The expression levels of heme oxygenase-1(HO-1) and inducible nitric oxide synthase(iNOS) in lung tissue were determined by immunohistochemistry.
Results The Results of HE staining in lung tissue showed that the lung injury in IR and A groups were more serious than that in group S, and the lung injury in group A was lighter than that in group IR.Compared with the group S, the ratio of W/D, MDA content, MPO activity and HO-1 and iNOS protein expression level significantly increased(P < 0.01), and the SOD activity significantly decreased in IR and A groups(P < 0.01).Compared with the group IR, the ratio of W/D, MDA content, MPO activity and iNOS protein expression level significantly decreased(P < 0.01), and the HO-1 protein level and SOD activity in lung tissue significantly increased in group A(P < 0.01).
Conclusions Anisodamine pretreatment can alleviate the lung injury induced by hepatic ischemia-reperfusion in rats.

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