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    杨青青, 张晓梅, 李辉, 张士荣, 周静, 严鸣. 高泌乳素血症中巨泌乳素筛查方法的研究[J]. 蚌埠医科大学学报, 2019, 44(1): 31-33. DOI: 10.13898/j.cnki.issn.1000-2200.2019.01.009
    引用本文: 杨青青, 张晓梅, 李辉, 张士荣, 周静, 严鸣. 高泌乳素血症中巨泌乳素筛查方法的研究[J]. 蚌埠医科大学学报, 2019, 44(1): 31-33. DOI: 10.13898/j.cnki.issn.1000-2200.2019.01.009
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    YANG Qing-qing, ZHANG Xiao-mei, LI Hui, ZHANG Shi-rong, ZHOU Jing, YAN Ming. Study on the screening method of macroprolactin in hyperprolactinemia[J]. Journal of Bengbu Medical University, 2019, 44(1): 31-33. DOI: 10.13898/j.cnki.issn.1000-2200.2019.01.009
    Citation: YANG Qing-qing, ZHANG Xiao-mei, LI Hui, ZHANG Shi-rong, ZHOU Jing, YAN Ming. Study on the screening method of macroprolactin in hyperprolactinemia[J]. Journal of Bengbu Medical University, 2019, 44(1): 31-33. DOI: 10.13898/j.cnki.issn.1000-2200.2019.01.009
    shu

    高泌乳素血症中巨泌乳素筛查方法的研究

    Study on the screening method of macroprolactin in hyperprolactinemia

      摘要
    • 摘要:
      目的探讨高泌乳素血症中巨泌乳素(M-PRL)对泌乳素(PRL)检测的干扰及应用血清稀释聚乙二醇(PEG)沉淀法筛查M-PRL血症(MP)的可行性。
      方法收集84例PRL>30.0 ng/mL病人血清标本，分别使用25%PEG沉淀法及25%PEG沉淀5倍稀释后的血清标本法测定高泌乳素血症病人的PRL水平，以回收率 < 50%为判断MP的临界值。采用ROC曲线评价血清稀释PEG沉淀法和PEG沉淀法对MP的诊断价值。
      结果使用25%PEG沉淀5倍稀释后的血清标本法对真泌组和巨泌组的PRL回收率分别是116.0%和63.0%，分别高于传统的PEG沉淀法中PRL的回收率的42.0%和101.0%(P < 0.05和P < 0.01)；且血清稀释PEG沉淀法对真性高泌乳素血症检出率98.53%，高于PEG沉淀法的检出率88.23%(P < 0.05)。血清稀释PEG沉淀法和PEG沉淀法诊断MP的ROC曲线下面积分别为0.939 (0.851~1.0)和0.932(0.828~1.0)。
      结论采用25% PEG沉淀5倍稀释后的血清标本法可以增加PRL的回收率，提高真性高泌乳素血症的检出率。

       

      Abstract:
      ObjectiveTo explore the effects of macroprolactin on the detection of prolactin(PRL), and the feasibility of the serum dilution polyethylene glycol(PEG) precipitation method in the screening of hyperprolactinemia(MP).
      MethodsThe PRL levels in blood samples of 84 patients with serum PRL level >30.0 ng/mL were detected using the 5-fold dilution 25% PEG and PEG precipitation method.The recovery rate of PRL less than 50% was used as the standard for MP screening.The value of 25% PEG and 5-fold dilution 25% PEG in the diagnosis of MP were evaluated using ROC curve.
      ResultsThe recovery rates of PRL in the eutrophic and hypertrophic groups were 116.0% and 63.0% using 5-fold dilution PEG precipitation method, and which was higher than those in traditional PEG precipitation method(42.0% and 101.0%), respectively(P < 0.05 and P < 0.01).The detection rate of dilution PEG precipitation method in genuine MP was 98.53%, which was higher than that of PEG precipitation method(88.23%)(P < 0.05).The area under the ROC curve of dilution PEG and PEG precipitation methods in diagnosing MP were 0.939(0.851 to 1.0) and 0.932(0.828 to 1.0), respectively.
      ConclusionsThe 5-fold dilution PEG precipitation method can increase the recovery rate of PRL and detection rate of genuine MP.

       

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