金樱子总黄酮的不同萃取组分高效液相色谱法分析

吴剑峰; 李峰; 陈传平; 翟金霞

doi:10.13898/j.cnki.issn.1000-2200.2019.04.022

金樱子总黄酮的不同萃取组分高效液相色谱法分析

High performance liquid dromatography analysis of different extraction components of flavonoids from Lavigate Rosae Fructus

摘要

摘要:
目的探讨金樱子总黄酮的不同萃取组分的黄酮含量。
方法以金樱子为原料，用60%乙醇取金樱子总黄酮，分别用石油醚、三氯甲烷、乙酸乙酯、正丁醇萃取，回收有机溶剂获石油醚、三氯甲烷、乙酸乙酯、正丁醇及水等5个萃取相。采用高效液相色谱-紫外检测法测定各相在不同波长下的峰值，并采用高效液相色谱-二极管阵列检测法分析各相中峰是否为黄酮。
结果结果显示小极性黄酮主要分布在三氯甲烷相中，占总黄酮的47.72%；大极性黄酮主要分布在正丁醇相中，占总黄酮的44.07%；而水相中有少量黄酮，占总黄酮的9.17%；石油醚相和乙酸乙酯相黄酮含量极少。
结论金樱子黄酮类成分主要富集于三氯甲烷相和正丁醇相。

Abstract:
ObjectiveTo analyze the flavonoids content in different extraction components from Laevigatae Rosae Fructus.
MethodsThe crude flavonoid was extracted from Rosae Laevigatae Fructus using 60% ethanol(v:v), and extracted using petroleum ether, chloroform, ethyl acetate and n-butanol.The five phases of petroleum ether, chloroform, ethyl acetate, n-butanol and water were obtained, the peak value of each phase was measured using HPLC-UV, and the peak of each phase was analyzed by HPLC-DAD.
ResultsThe low polar flavonoids mainly distributed in the chloroform phase, which accounted for 47.72% of the total flavonoids.The high polar flavonoids mainly distributed in the n-butanol phase, which accounted for 44.07% of the total flavonoids.The small amount of flavonoids in water phase was identified, which accounted for 9.17% of the total flavonoids.The very small amount of flavonoids in petroleum ether and ethyl acetate phases was found.
ConclusionsThe flavonoids of Laevigate Rosae Fructus mainly distributes in the phase of trichloromethane and n-butanol.

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