内皮祖细胞在裸鼠体内再现人血管瘤的生物学特性研究

吴军军; 张莉; 蒋邦红; 霍继武; 宋培军

doi:10.13898/j.cnki.issn.1000-2200.2019.05.002

内皮祖细胞在裸鼠体内再现人血管瘤的生物学特性研究

Biological characteristics of the endothelial progenitor cells in regenerating human hemangioma in nude mice

摘要

摘要:
目的研究人血管瘤内皮祖细胞（EPcs）在裸鼠体内分化情况，进一步揭示其生物学特性。
方法组织块法体外培养增生期血管瘤内皮祖细胞（经CD31、CD34及Ⅷ因子相关抗原鉴定），浓集后分别种植于裸鼠双侧胸壁、腰部皮下，动态观察移植瘤生长情况及组织学变化，并用血管内皮细胞特异性标志物鼠抗人CD31、CD34免疫组化单克隆抗体染色研究所成瘤体的同源性。
结果体外培养血管瘤内皮祖细胞生长良好，细胞计数为3×10⁸个/培养瓶，CD31、CD34及Ⅷ因子相关抗原免疫组化检测阳性表达，裸鼠皮下植入后所形成的瘤体与原血管瘤生物学特点及病理特征相似，经CD31、CD34鉴定瘤体来源于人血管瘤内皮细胞。
结论内皮祖细胞是血管瘤发生的细胞来源，此可作为血管瘤的动物模型，为血管瘤的研究提供较理想平台。

Abstract:
ObjectiveTo study the differentiation of human hemangioma endothelial progenitor cells(EPcs) in nude mice, and further reveal its biological characteristics.
MethodsThe hemangioma endothelial progenitor cells in proliferative stage were cultured in vitro, identified by CD31, CD34 and Ⅷ-factor related antigens, and subcutaneously inoculated into the bilateral chest wall and waist of nude mice.The growth characteristics and histologic changes of transplanted tumors were regularly observed.The transplanted tumor's homology was identified by immunohistochemical staining of the vascular endothelial cell specific marker CD31 and CD34.
ResultsThe growth of EPcs was good in vitro, the number of EPcs was 3×10⁸, and the positive expressions of CD31, CD34 and Ⅷ-factor related antigen were detected by immunohistochemistry.The biological and pathological characteristics between tumor formation after subcutaneous implantation and original hemangioma were similar in nude mice.The tumor identified by CD31 and CD34 was derived from human hemangioma endothelial cells.
ConclusionsThe cell source of hemangioma is derived from EPCs, which can be used as the animal models of hemangioma, and provide an ideal platform for the study of hemangioma.

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