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    李燕, 柯希权, 邓敏, 李大鹏, 汪强武, 汪建超, 王启之. 星形胶质细胞上调基因-1对胃癌干细胞增殖、凋亡及干性的影响[J]. 蚌埠医科大学学报, 2019, 44(5): 570-577. DOI: 10.13898/j.cnki.issn.1000-2200.2019.05.003
    引用本文: 李燕, 柯希权, 邓敏, 李大鹏, 汪强武, 汪建超, 王启之. 星形胶质细胞上调基因-1对胃癌干细胞增殖、凋亡及干性的影响[J]. 蚌埠医科大学学报, 2019, 44(5): 570-577. DOI: 10.13898/j.cnki.issn.1000-2200.2019.05.003
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    LI Yan, KE Xi-quan, DENG Min, LI Da-peng, WANG Qiang-wu, WANG Jian-chao, WANG Qi-zhi. Effect of astrocyte elevated gene-1 on the proliferation, apoptosis and stemness of gastric cancer stem cells[J]. Journal of Bengbu Medical University, 2019, 44(5): 570-577. DOI: 10.13898/j.cnki.issn.1000-2200.2019.05.003
    Citation: LI Yan, KE Xi-quan, DENG Min, LI Da-peng, WANG Qiang-wu, WANG Jian-chao, WANG Qi-zhi. Effect of astrocyte elevated gene-1 on the proliferation, apoptosis and stemness of gastric cancer stem cells[J]. Journal of Bengbu Medical University, 2019, 44(5): 570-577. DOI: 10.13898/j.cnki.issn.1000-2200.2019.05.003
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    星形胶质细胞上调基因-1对胃癌干细胞增殖、凋亡及干性的影响

    Effect of astrocyte elevated gene-1 on the proliferation, apoptosis and stemness of gastric cancer stem cells

      摘要
    • 摘要:
      目的探讨星形胶质细胞上调基因-1（AEG-1）在胃癌干细胞中的表达及其作用。
      方法采用实时荧光定量技术（qRT-PCR）及Western blotting检测胃癌干细胞（HGC-27、MKN-45）及相应非干细胞mRNA及蛋白表达水平，采用瞬时转染法对AEG-1进行处理，使用MTS、细胞周期检测细胞增殖情况及作用机制，Annexin Ⅴ及Caspase-3/7活性检测细胞凋亡情况，干细胞成球实验评估AEG-1沉默对胃干细胞干性的影响，裸鼠体内成瘤实验评估AEG-1对裸鼠体内胃干细胞肿瘤发生的影响。Ⅴ
      结果胃癌干细胞MKN-45中AEG-1表达水平高于非干细胞组（P < 0.01）；AEG-1-siRNA转染的癌干细胞导致癌干细胞周期停滞在G1期，明显抑制了细胞增殖（P < 0.01）；Annexin Ⅴ及Caspase 3/7活性检测AEG-1敲低可诱导胃干细胞凋亡；AEG-1表达被敲低时显著抑制了胃癌干细胞的成球能力；AEG-1缺失抑制了肿瘤干细胞的体内成瘤能力。
      结论AEG-1沉默抑制细胞增殖，诱导胃癌干细胞G1期细胞周期停滞和细胞凋亡，抑制肿瘤发生，在胃癌干细胞的肿瘤发生中起着积极作用。

       

      Abstract:
      ObjectiveTo investigate the expression level and effects of astrocyte elevated gene-1(AEG-1) in gastric cancer stem cells.
      MethodsThe expression levels of AEG-1 mRNA and protein in gastric stem cell lines(HGC-27 and MKN-45) were detected using RT-PCR and Western blotting, respectively.The AEG-1 was processed using transient transfection, and the cells proliferation and its mechanism were investigated using the MTS assay and cell-cycle analysis.The apoptosis was examined using the activities of Caspase-3/7 and annexin V, the effect of silencing AEG-1 on the stemness of gastric stem cells was evaluated using the tumorsphere formation assay, and the tumor formation experiment was conducted to evaluate the effects of AEG-1 on the tumorigenesis of gastric stem cells in nude mice.
      ResultsThe expression level of AEG-1 in gastric stem cells was higher than that in non-stem cells(P < 0.01).Cancer stem cells transfected with AEG1-siRNA resulted in the cancer stem cell cycle arrest in G1 phase, and could significantly inhibit the cell proliferation(P < 0.01).The results of Annexin V and Caspase-3/7 activities detection showed that the knockdown of AEG-1 could induce the apoptosis of gastric stem cells.The low level of AEG-1 could significantly inhibit the ability of gastric cancer stem cells to form into balls.The loss of AEG-1 inhibited the tumorigenesis of tumor stem cells in vivo.
      ConclusionsThe silencing expression of AEG-1 can suppress the cell proliferation, induce the cell cycle arrest in G1 phase and apoptosis of gastric cancer stem cells, inhibit the tumorigenesis, which plays a positive role in tumorigenesis of gastric cancer stem cells.

       

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