焦虑状态下去甲肾上腺素激活Akt信号通路对乳腺癌作用研究

欧阳雪岩; 朱朕; 杨超; 王丽; 姜峰; 丁罡

doi:10.13898/j.cnki.issn.1000-2200.2019.07.001

焦虑状态下去甲肾上腺素激活Akt信号通路对乳腺癌作用研究

Study on norepinephrine activating Akt signaling pathway in breast cancer under anxiety state

摘要

摘要:
目的探讨去甲肾上腺素在焦虑状态下对乳腺癌的作用及机制。
方法将20只C57BL/6小鼠分为对照组及模型组，通过接种肿瘤细胞和实验刺激建立焦虑荷瘤小鼠模型，造模结束后检测血清中去甲肾上腺素水平并测量肿瘤体积。人乳腺癌细胞系MDA-MB-231细胞在去甲肾上腺素处理后检测其增殖、迁移、侵袭、细胞周期分布及Akt信号通路的激活状态。
结果模型组小鼠肿瘤体积大于对照组（P < 0.05），血清中去甲肾上腺素含量明显升高（P < 0.01），且同焦虑程度呈现相关关系（r=0.78）。去甲肾上腺素提高了MDA-MB-231细胞的增殖、迁移和侵袭能力，并且促进DNA复制以及Akt信号通路的激活。
结论去甲肾上腺素在焦虑状态下激活AKt信号通路，促进乳腺癌的发展。

Abstract:
ObjectiveTo explore the effects and mechanism of norepinephrine in breast cancer under anxiety state.
MethodsTwenty C57BL/6 mice were divided into the control group and model group.The mice models with anxiety tumor-bearing were established using inoculation of tumor cells and experimental stimulation.The serum level of norepinephrine was detected, and the tumor volume was measured after modeling.The proliferation, migration, invasion, cell cycle distribution and activation of Akt signaling pathway of MDA-MB-231 breast cancer cells treated with norepinephrine were detected.
ResultsThe tumor volume in model group was significantly larger than that in control group (P < 0.05), and the serum concentration of norepinephrine significantly increased (P < 0.01), which was correlated with the anxiety degree (r=0.78).Norepinephrine enhanced the proliferation, migration and invasion of MDA-MB-231 cells, promoted DNA replication, and activated the Akt signaling pathway.
ConclusionsNorepinephrine activates the Akt signaling pathway to promote the development of breast cancer under state anxiety.

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