SOX7基因对肝癌细胞凋亡、氧化应激及Wnt/β-catenin信号通路的影响

冯桂银; 张庚; 王海伦; 梁东启; 袁媛; 樊艳

doi:10.13898/j.cnki.issn.1000-2200.2019.07.002

SOX7基因对肝癌细胞凋亡、氧化应激及Wnt/β-catenin信号通路的影响

Effect of SOX7 gene on the apoptosis, oxidative stress and Wnt/β-catenin signaling pathway in liver cancer cells

摘要

摘要:
目的探讨过表达SOX7对肝癌细胞凋亡、活性氧（ROS）水平及Wnt/β-catenin信号通路的影响。
方法人肝癌Huh-7细胞分为空白组、空质粒pcDNA3.1组（Vector组）和pcDNA3.1-SOX7重组质粒组（pcDNA3.1-SOX7组）。转染48 h，Annexin V-FITC/PI双染法细胞凋亡试剂盒、DCFH-DA法及Western blotting分别检测细胞凋亡率、ROS含量及SOX7、β-catenin、cyclin D1和cleaved-caspase 3蛋白表达。MTT法检测pcDNA3.1转染24 h、48 h和72 h的细胞活力。
结果pcDNA3.1-SOX7重组质粒组SOX7蛋白表达明显高于空白组（P < 0.05）。与空白组比较，pcDNA3.1-SOX7组细胞活力及β-catenin和cyclin D1蛋白表达均明显降低，细胞凋亡率、ROS含量及cleaved-caspase 3蛋白表达明显升高（P < 0.05）。
结论过表达SOX7可诱导肝癌细胞凋亡，凋亡机制与细胞内ROS水平提高及Wnt/β-catenin信号通路抑制有关。

Abstract:
ObjectiveTo investigate the effects of overexpression of SOX7 gene on apoptosis, ractive oxygen species (ROS) level and Wnt/β-catenin signaling pathway in hepatocellular carcinoma (HCC) cells.
MethodsThe human hepatoma Huh-7 cells were divided into the blank group, blank plasmid pcDNA3.1 group (vector group) and pcDNA3.1-SOX7 recombinant plasmid group (pcDNA3.1-SOX7 group).The apoptosis rate, ROS content, and expression levels of SOX7, β-catenin, cyclin D1 and cleaved-caspase 3 protein were detected by Annexin V-FITC/PI double staining, DCFH-DA assay and Western blotting at 48 h after transfection, respectively.The cell viability was detected using MTT assay after 24 h, 48 h and 72 h of pcDNA3.1 transfecting.
ResultsThe expression level of SOX7 protein in pcDNA3.1-SOX7 recombinant plasmid group was significantly higher than that in blank group (P < 0.05).Compared with the blank group, the cell viability and expression levels of β-catenin and cyclin D1 protein in pcDNA3.1-SOX7 group significantly decreased, while the apoptosis rate, ROS content and cleaved-caspase 3 protein expression level significantly increased (P < 0.05).
ConclusionsThe overexpression of SOX7 can induce the apoptosis of HCC cells.The mechanism of apoptosis is related to the increasing of ROS level and inhibition of Wnt/β-catenin signaling pathway.

HTML全文

参考文献(21)

施引文献

资源附件(0)