长链非编码RNA-ROR对结直肠癌SW620细胞增殖的影响

施炜; 胡世丰; 雷春; 吴伙

doi:10.13898/j.cnki.issn.1000-2200.2019.10.001

长链非编码RNA-ROR对结直肠癌SW620细胞增殖的影响

Effect of LncRNA-ROR on the proliferation of colorectal cancer SW620 cells

摘要

摘要:
目的了解长链非编码RNA-ROR（LncRNA-ROR）在结直肠癌SW620细胞增殖中的作用。
方法选择40例结直肠癌组织及相应正常组织（距肿瘤切缘5cm以外），首先采用qRT-PCR技术检测LncRNA-ROR在结直肠癌组织中的表达情况，并分析其表达与病人临床病理因素的相关性。构建慢病毒载体LV5-ROR、LV5-Vector转染到结直肠癌细胞SW620中，验证LncRNA-ROR的表达，分析LncRNA-ROR过表达对结直肠癌细胞CCK-8功能及SW620平板克隆形成的影响。
结果与正常组织比较，LncRNA-ROR在结直肠癌组织中低表达（P < 0.01），其表达水平与TNM分期具有相关性（P < 0.05）。经转染筛选后，qRT-PCR检测证实SW620中的LncRNA-ROR表达增加，其高表达导致SW620增殖能力的下降（P < 0.01）。
结论LncRNA-ROR的过表达抑制了结直肠癌细胞SW620的增殖。

Abstract:
ObjectiveTo investigate the effects of long non-coding RNA-ROR(LncRNA-ROR) on the proliferation of colorectal cancer SW620 cells.
MethodsThe expression level of LncRNA-ROR in colorectal cancer tissue was detected using qRT-PCR, and the relationship between the expression level of LncRNA-ROR and clinicopathological data was analyzed.The lentiviral vector LV5-ROR was constructed, and transfected into the colorectal cancer SW620 cells and verified.The effects of LncRNA-ROR overexpression on the function and colony formation of SW620 cells were analyzed.
ResultsCompared with the normal tissue, the expression level of LncRNA-ROR was low in colorectal cancer tissue(P < 0.01), and its expression level was closely related to TNM stage(P < 0.05).After transfection, the expression level of LncRNA-ROR increasing in SW620 cells was identified by qRT-PCR, and its high expression led to the decreasing of proliferation function of SW620 cells(P < 0.01).
ConclusionsThe overexpression of LncRNA-ROR can inhibit the proliferation of SW620 cells.

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