三阴性乳腺癌的上皮间质转化及自噬特性差异的研究

冯琛; 朱超; 余定玥; 刘蕴霞; 宋早智; 王庆康; 钱军

doi:10.13898/j.cnki.issn.1000-2200.2019.11.003

三阴性乳腺癌的上皮间质转化及自噬特性差异的研究

Study on the difference between epithelial-mesenchymal transformation and autophagy in triple negative breast cancer

摘要

摘要:
目的探讨三阴性乳腺癌（TNBC）的上皮间质转化（EMT）及自噬特性的差异。
方法选取TNBC细胞株BT-549与非TNBC细胞株MCF-7，分别对比其EMT及自噬特性。通过细胞形态，平板克隆，Transwell侵袭实验，Western blotting实验测定其EMT的差异，通过电镜观察自噬体，免疫荧光实验，Western blotting实验测定其自噬特性。
结果TNBC增殖性和侵袭性强于非TNBC，TNBC组间质细胞标志物N-cadherin、Vimentin、β-catenin相对高表达，非TNBC组上皮细胞标志物E-cadherin相对高表达。电镜下，非TNBC细胞线粒体自噬，TNBC组自噬微管相关蛋白1轻链3相对高表达。
结论TNBC增殖性、侵袭性皆强于非TNBC，EMT及自噬化程度均较高。

Abstract:
ObjectiveTo explore the difference between epithelial-mesenchymal transition(EMT) and autophagy properties in triple negative breast cancer.
MethodsThe epithelial-mesenchymal transition(EMT) and autophagy characteristics were compared between three negative breast cancer(TNBC) cell line BT-549 and non-triple negative breast cancer(non-TNBC) cell line MCF-7.The EMT were investigated using the cell morphology, plate cloning, transwell invasion assay and western blot analysis.The autophagy characteristics were observed by electron microscopy, immunofluorescence assay and western blot analysis.
ResultsThe proliferation and invasion in TNBC were stronger than that in non-TNBC.The N-cadherin, Vimentin and β-catenin of interstitial cell markers were relatively high expression in TNBC group, and the E-cadherin of epithelial cell markers was relatively high expression in non-TNBC group.Under electron microscope, the mitochondrial autophagy was detected in non-TNBC cells, and the autophagy microtubule associated protein 1 light chain 3 was relatively high expression in TNBC group.
ConclusionsThe proliferation and invasion in TNBC are stronger than that in non-TNBC, and the EMT and degree of autophagy are high in TNBC.

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