高糖对人视网膜色素上皮细胞syndecan-1表达的影响

韩小霞; 王静波

doi:10.13898/j.cnki.issn.1000-2200.2019.12.004

高糖对人视网膜色素上皮细胞syndecan-1表达的影响

Effect of high glucose on the expression level of syndecan-1 in human retinal pigment epithelial cells

摘要

摘要:
目的检测高糖对视网膜色素上皮（RPE）细胞syndecan-1表达的影响。
方法25 mmol/L高糖刺激人RPE细胞4 h、12 h和24 h，分别采用免疫组织化学和蛋白印迹法检测细胞syndecan-1蛋白的表达，采用实时定量聚合酶链反应检测syndecan-1 mRNA的表达。
结果高糖刺激后，RPE细胞排列紊乱，细胞体变长变大，脱落细胞多见。正常RPE细胞表达syndecan-1，且定位于细胞质。高糖刺激4 h，RPE细胞syndecan-1的蛋白和mRNA表达开始下降，12 h时表达降至最低，24 h时syndecan-1的表达基本回复至正常水平（P < 0.01）。
结论高糖可短暂下调RPE细胞syndecan-1蛋白和mRNA的表达。

Abstract:
ObjectiveTo detect the effects of high glucose on the expression level of syndecan-1 in human retinal pigment epithelial(RPE) cells.
MethodsThe RPE cells were treated with 25 mmol/L glucose for 4 h, 12 h and 24 h, respectively.The protein and mRNA expression levels of syndecan-1 were detected using immunohistochemistry staining and Western blot, and quantitative real-time polymerase chain reaction(qPCR), respectively.
ResultsAfter exposure to high glucose, the RPE cells were arranged in disorder, the cell bodies became longer and larger, the shedding cells were easily seen.The syndecan-1 expression was detected in normal RPE cells, and located in cytoplasm.After 4 h of treatment with high glucose, the expression levels of syndecan-1 protein and mRNA decreased, decreased to the minimum at 12 h, and basically returned to the normal level at 24 h(P < 0.01).
ConclusionsHigh glucose can transiently down-regulate the syndecan-1 protein and mRNA expression in RPE cells.

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