CX26、CX32、CX43与上皮间质转化相关蛋白在结直肠原发癌和肝脏转移之间的相关性

张露; 杨燕; 苏方

doi:10.13898/j.cnki.issn.1000-2200.2019.12.012

CX26、CX32、CX43与上皮间质转化相关蛋白在结直肠原发癌和肝脏转移之间的相关性

Study on the correlation between the levels of CX26, CX32, CX43 and epithelial mesenchymal transformation-associated protein, and colorectal primary carcinoma and liver metastasis

摘要

摘要:
目的探讨间隙连接蛋白（CX）26、CX32、CX43与上皮间质转化相关蛋白上皮型钙黏蛋白（E-cadherin）、神经型钙黏蛋白（N-cadherin）在结直肠原发癌和肝脏转移之间的相关性。
方法选取初诊时已发生结直肠癌肝脏转移并手术治疗者40例，经手术治疗病理确诊为结直肠癌肝转移石蜡标本40例，以上研究对象分为3组：A组为结、直肠癌组织40例，B组为癌旁正常组织40例，C组为肝转移灶40例，所有病人术前均未行化疗、放射、介入及免疫治疗。利用免疫组织化学SP法检测E-cadherin、N-cadherin及CX26、CX32、CX43的表达。分析E-cadherin、N-cadherin及CX26、CX32、CX43表达与结直肠癌肝脏转移的临床病理特征（性别、年龄、肿瘤浸润深度、组织分化程度、淋巴结转移情况）的关系；及结直肠癌发生肝脏转移和上皮间质转化及细胞间隙蛋白CX26、CX32、CX43的相关性。
结果 3组除CX43蛋白阳性表达率差异有统计学意义（P < 0.05）外，CX26、CX32、E-cadherin和N-cadherin蛋白阳性表达率差异均无统计学意义（P>0.05）；E-cadherin、N-cadherin及CX26、CX32、CX43与病人的性别、年龄、肿瘤浸润深度及淋巴结转移情况差异均无统计学意义（P>0.05），而N-cadherin与组织分化程度呈明显相关关系（P < 0.05）。
结论 CX43、N-cadherin与结直肠原发癌和肝脏转移有关。

Abstract:
Objective To investigate the correlation between connexin(CX) 26, CX32, CX43 and epithelial mesenchymal transformation associated protein(E-cadherin and N-cadherin), and colorectal primary carcinoma and liver metastasis.
Methods Forty colorectal cancer patients with liver metastasis diagnosed by pathological examination were investigated, and treated without chemotherapy, radiation, intervention and immunity therapy before operation.Forty colorectal cancer tissue specimens, 40 normal tissues adjacent to cancer specimens and 40 liver metastasis tissue specimens were divided into the group A, group B and group C, respectively.The expression levels of E-cadherin, N-cadherin, CX26, CX32 and CX43 in three groups were detected by immunohistochemical SP method.The relationship between the expression levels of E-cadherin, N-cadherin, CX26, CX32 and CX43 and clinicopathological features(including gender, age, depth of tumor invasion, degree of tissue differentiation and lymph node metastasis) of colorectal cancer were analyzed.The correlations of liver metastasis with levels of epithelial mesenchymal transformation, intercellular protein, CX26, CX32 and CX43 in colorectal cancer were analyzed.
Results The differences of the positive expression rate of CX43 among the three groups were statistically significant(P < 0.05), and the differences of the positive expression rate of CX26, CX32, E-cadherin and N-cadherin among three groups were not statistically significant(P>0.05).The differences of the expression levels of E-cadherin, E-cadherin, CX26, CX32 and CX43 in different sex, age, depth of tumor invasion, degree of tissue differentiation and lymph node metastasis were not statistically significant(P>0.05), and the expression level of N-cadherin was positively correlated with degree of tissue differentiation(P < 0.05).
Conclusions The expression levels of CX43 and epithelial mesenchymal transformation-associated proteins are significantly associated with colorectal primary carcinoma and liver metastasis.

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