ObjectiveTo investigate the changes of the serum levels of tumor necrosis factor-α (TNF-α), hypersensitive Creactive protein (hs-CRP) and high mobility protein B1 (HMGB1) in patients with type 2 diabetes mellitus complicated with coronary heart disease, and its clinical significance.

MethodsThirty-two patients with type 2 diabetes mellitus, 38 patients with coronary artery disease, 40 patients with type 2 diabetes mellitus complicated with coronary artery disease, and 40 healthy examiners were divided into group A, group B, group C and control group, respectively.The levels of TNF-α, hs-CRP and HMGB1 were measured by ELISA and scatter turbidimetric method.

ResultsThe differences of the serum levels of hs-CRP, TNF-α and HMGB1 among four groups were statistically significant (P < 0.01), which in group C was significantly higher than that in group B, which in group B was significantly higher than that in group A, and which in group A was significantly higher than that in control group (P < 0.01).With the number of coronary artery lesions increasing, the serum levels of TNF-α, hs-CRP and HMGB1 increased, the differences of the serum levels of TNF-α, hs-CRP and HMGB1 between single-branch lesion group and double-branch lesion group were not statistically significant (P>0.05), and the differences of various indexes among other groups were statistically significant (P < 0.01).After treatment, the serum level of FPG was positively correlated with hs-CRP and TNF-α levels (P < 0.01), the serum levels of HMGB1 and hs-CRP were positively correlated with TNF-α level (P < 0.05), and the Gensini score was positively correlated with the serum level of HMGB1 (P < 0.05).

ConclusionsTNF-α, hs-CRP and HMGB1 are all involved in the occurrence and development of atherosclerosis in patients with type 2 diabetes mellitus complicated with coronary heart disease.The expression levels of three indicators have guiding significance in the diagnosis of disease and treatment of patients.