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    王振杰, 徐志鹏, 陈硬, 宋琦. 醋酸钠林格液对失血性休克大鼠心肌炎性介质及NF-κB、MAPK信号通路的影响[J]. 蚌埠医科大学学报, 2020, 45(3): 281-285. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.001
    引用本文: 王振杰, 徐志鹏, 陈硬, 宋琦. 醋酸钠林格液对失血性休克大鼠心肌炎性介质及NF-κB、MAPK信号通路的影响[J]. 蚌埠医科大学学报, 2020, 45(3): 281-285. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.001
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    WANG Zhen-jie, XU Zhi-peng, CHEN Ying, SONG Qi. Effect of sodium acetate Ringer's solution on inflammatory mediators and NF-κB, MAPK signaling pathway in myocardial tissue of hemorrhagic shock rats[J]. Journal of Bengbu Medical University, 2020, 45(3): 281-285. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.001
    Citation: WANG Zhen-jie, XU Zhi-peng, CHEN Ying, SONG Qi. Effect of sodium acetate Ringer's solution on inflammatory mediators and NF-κB, MAPK signaling pathway in myocardial tissue of hemorrhagic shock rats[J]. Journal of Bengbu Medical University, 2020, 45(3): 281-285. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.001
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    醋酸钠林格液对失血性休克大鼠心肌炎性介质及NF-κB、MAPK信号通路的影响

    Effect of sodium acetate Ringer's solution on inflammatory mediators and NF-κB, MAPK signaling pathway in myocardial tissue of hemorrhagic shock rats

      摘要
    • 摘要:
      目的探讨醋酸钠林格液复苏创伤失血性休克大鼠对其心肌炎性介质及NF-κB、MAPK信号通路的影响。
      方法健康SD大鼠32只，随机分为失血性休克未复苏组（CR组）、0.9%氯化钠溶液复苏组（NR组）、乳酸钠林格液复苏组（LR组）和醋酸钠林格液复苏组（AR组），各8只。各组大鼠制备休克模型（平均动脉压维持30 mmHg），CR组不予复苏，NR组、LR组及AR组于休克后60 min应用相应液体进行复苏，复苏后观察4 h。取各组大鼠心肌组织，进行心肌组织形态学观察，并检测相关细胞因子及蛋白表达水平。
      结果AR组、LR组、NR组大鼠心肌组织TNF-α mRNA表达水平均明显低于CR组（P < 0.01），AR组和LR组IL-10 mRNA均高于CR组（P < 0.01和P < 0.05），AR组IL-4 mRNA均明显高于LR组、NR组和CR组（P < 0.01）。AR组、LR组、NR组大鼠p65（Ser536）磷酸化、p65（Lys310）乙酰化、JNK磷酸化蛋白相对表达量均低于CR组（P < 0.05~P < 0.01），且AR组均低于NR组和LR组（P < 0.05~P < 0.01）；AR组、LR组、NR组MKP-1乙酰化蛋白相对表达量均高于CR组（P < 0.05~P < 0.01），且AR组和LR组均明显高于CR组（P < 0.01）。
      结论醋酸钠林格液复苏失血性休克大鼠可能通过调控NF-κB和MAPK信号的表达保护心肌组织，在一定程度上减轻心肌组织炎症反应。

       

      Abstract:
      ObjectiveTo explore the effect of sodium acetate Ringer's solution on inflammatory mediators and NF-κB, MAPK signaling pathway in myocardial tissue of traumatic hemorrhagic shock rats.
      MethodsThirty two healthy SD rats were randomly divided into shock without resuscitation group (CR group), resuscitation with 0.9% sodium chloride solution group (NR group), resuscitation with sodium lactate Ringer's solution group (LR group), and resuscitation with sodium acetate Ringer's solution group (AR group) (eight rats in each group).The shock model was made in each group (the average arterial pressure maintained at 30 mmHg).CR group was not resuscitated, NR, LR and AR group were resuscitated with corresponding solution at 60 min after shock, and observed for 4 h after resuscitation.The myocardial tissue of rats in each group was taken for morphological observation, and the expression level of related cytokines and proteins was detected.
      ResultsThe expression level of TNF-α mRNA of myocardial tissue in AR, LR and NR group was significantly lower than that in CR group (P < 0.01), the expression level of IL-10 mRNA in AR and LR group was significantly higher than that in CR group (P < 0.01 and P < 0.05), and the expression level of IL-4 mRNA in AR group was significantly higher than that in LR, NR and CR group (P < 0.01).The relative expression of p65 (Ser536) phosphorylation, p65(Lys310) acetylation and JNK phosphorylation protein in AR, LR and NR group was lower than that in CR group (P < 0.05 to P < 0.01), and which in AR group was lower than that in NR and LR group (P < 0.05 to P < 0.01).The relative expression of MKP-1 acetylation protein in AR, LR and NR group was higher than that in CR group (P < 0.05 to P < 0.01), and which in AR and LR group was significantly higher than that in CR group (P < 0.01).
      ConclusionsSudium acetate Ringer's solution for resuscitating hemorrhagic shock rats may protect myocardial tissue by regulating the expression of NF-κB and MAPK signaling pathway, and reduce the inflammatory response of myocardial tissue to a certain extent.

       

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