Abstract:
ObjectiveTo investigate the effect and clinical significance of different fluid resuscitation in patients with hemorrhagic shock on the percentage of helper T cell 17(Th17) and regulatory T cell(Treg) in peripheral blood and related cytokines, and to provide reference for clinical screening of relatively ideal resuscitation fluid.
MethodsFifty-six patients with traumatic hemorrhagic shock were enrolled as the trauma group.All patients were randomly divided into sodium acetate Ringer's solution (AR) group.And the sodium lactate Ringer's solution (LR) group, 28 cases in each, 18 cases of our hospital health physician volunteers were selected as the control group.Peripheral blood was collected and the ratio of Th17 and Treg in peripheral blood was detected by flow cytometry.The levels of IL-17, IL-6 and IL-23 TNF-α level in peripheral blood of each group were detected by enzyme-linked immunosorbent assay (ELISA).
ResultsCompared with the control group, the peripheral blood Th17 and Treg ratios and the levels of IL-17, IL-6, IL-23 and TNF-α in the trauma group (LR group+AR group) were significantly increased (P < 0.01).Before resuscitation, there was no significant difference in the peripheral blood Th17, Treg ratio and cytokine levels between the two groups of patients in the trauma group (P>0.05).Thirty minutes after resuscitation, the increase in Th17 ratio and IL-6 and TNF-α levels in the LR group were greater than those in the AR group (P < 0.05 to P < 0.01);there was no significant difference in the level of Treg ratio, IL-17, and IL-23 in the two groups(P>0.05).One hour after resuscitation, there was no statistically significant difference in the changes of the two groups (P>0.05).
ConclusionsCompared with sodium lactate Ringer's solution, the early resuscitation with sodium acetate Ringer's solution further inhibits the release of Th17 cells and IL-6 and TNF-α in peripheral blood of patients with traumatic hemorrhagic shock, thereby reducing the patient's body inflammation.
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