N-乙酰半胱氨酸对乙醇诱导张氏肝细胞损伤的保护作用

陈苏; 谢丽丽; 崔慧娴; 赵文红

doi:10.13898/j.cnki.issn.1000-2200.2020.03.006

N-乙酰半胱氨酸对乙醇诱导张氏肝细胞损伤的保护作用

Protective effects of NAC on the ethanol-induced Chang hepatocellular injury

摘要

摘要:
目的探讨N-乙酰半胱氨酸（NAC）对体外乙醇处理诱导的张氏肝细胞损伤的保护作用。
方法体外培养张氏肝细胞，实验分为对照组、乙醇处理组和NAC高、中、低剂量进行预处理的保护组，并测定NAC预处理对乙醇诱导的肝细胞损伤模型的细胞存活率，细胞内天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、活性氧（ROS）、细胞上清液中肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）水平的影响，评价NAC是否对乙醇造成的肝细胞损伤具有保护作用。
结果NAC预处理后，细胞的存活率高于乙醇处理组（P < 0.05），细胞形态学观察结果显示，NAC的干预组细胞形态优于乙醇处理组；NAC预处理显著降低细胞内AST、ALT、ROS和细胞上清液中TNF-α和IL-6的含量（P < 0.05）。
结论NAC干预可减轻体外乙醇处理诱导的肝细胞损伤，并降低细胞内ROS和炎性因子的水平。

Abstract:
ObjectiveTo investigate the protective effects of N-acetylcysteine(NAC) on ethanol-induced Chang hepatocellular injury.
MethodsThe Chang hepatocytes were cultured in vitro, and divided into the control group, ethanol treatment group, and high, medium and low dose NAC protection groups.The cell survival rate of ethanol-induced hepatocellulars injury model by NAC pretreatment was determined, and the effects of aspartate aminotransferase(AST), alanine aminotransferase(ALT), intracellular reactive oxygen species(ROS), tumor necrosis factor-α(TNF-α), and interleukin-6(IL-6) were evaluated.The protective effects of NAC on ethanol-induced Chang hepatocellular injury were analyzed.
ResultsAfter NAC pretreatment, the cell survival rate was higher than that of the ethanol-treated group(P < 0.05).The results of cell morphology showed that the cellular morphology in NAC intervention group was better than that in ethanol-treated group.The NAC pretreatment could significantly reduce the contents of TNF-α and IL-6 in cell supernatant, and levels of AST, ALT and ROS in cells(P < 0.05).
ConclusionsThe NAC intervention can alleviate the Chang hepatocellular injury induced by ethanol treatment in vitro, and reduce the levels of intracellular ROS and inflammatory factors.

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