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    刘红艳, 齐创. 萝卜硫素调控TGF-β1/Smad信号通路抑制结肠癌HT-29细胞增殖的机制研究[J]. 蚌埠医科大学学报, 2020, 45(3): 311-314. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.007
    引用本文: 刘红艳, 齐创. 萝卜硫素调控TGF-β1/Smad信号通路抑制结肠癌HT-29细胞增殖的机制研究[J]. 蚌埠医科大学学报, 2020, 45(3): 311-314. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.007
    shu
    LIU Hong-yan, QI Chuang. Sulforaphane inhibits proliferation of colon cancer HT-29 cells via regulating TGF β1/Smad signaling pathway[J]. Journal of Bengbu Medical University, 2020, 45(3): 311-314. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.007
    Citation: LIU Hong-yan, QI Chuang. Sulforaphane inhibits proliferation of colon cancer HT-29 cells via regulating TGF β1/Smad signaling pathway[J]. Journal of Bengbu Medical University, 2020, 45(3): 311-314. DOI: 10.13898/j.cnki.issn.1000-2200.2020.03.007
    shu

    萝卜硫素调控TGF-β1/Smad信号通路抑制结肠癌HT-29细胞增殖的机制研究

    Sulforaphane inhibits proliferation of colon cancer HT-29 cells via regulating TGF β1/Smad signaling pathway

      摘要
    • 摘要:
      目的研究萝卜硫素对结肠癌HT-29细胞增殖的影响及其可能的作用机制。
      方法采用CCK8分析萝卜硫素对HT-29细胞增殖的影响，流式细胞术检测HT-29细胞凋亡，ELISA检测细胞培养液中转化生长因子β1（TGF-β1）的水平，Western blotting检测细胞中p-Smad3、Smad4、Cyclin D1及c-Myc蛋白表达。
      结果CCK8结果显示，随着药物浓度和作用时间的增加，萝卜硫素对HT-29细胞的增殖抑制作用增强（P < 0.05~P < 0.01）。10、20、40 μmol/L萝卜硫素干预HT-29细胞24 h，细胞凋亡率均高于对照组（P < 0.05~P < 0.01）。ELISA结果表明，药物处理HT-29细胞24 h，10、20、40 μmol/L萝卜硫素组细胞培养液中TGF β1水平均低于对照组（P < 0.05~P < 0.01）。与对照组相比，10、20、40 μmol/L萝卜硫素均能抑制p-Smad3、Smad4、Cyclin D1及c-Myc蛋白表达（P < 0.05~P < 0.01）。
      结论萝卜硫素可抑制结肠癌HT-29细胞的增殖，其作用机制可能与调控TGF-β1/Smad信号通路有关。

       

      Abstract:
      ObjectiveTo study the effect of sulforaphane on the proliferation of colon cancer HT-29 cells and its potential mechanism.
      MethodsCCK8 was used to detect the effect of sulforaphane on the proliferation of HT-29 cells.Flow cytometry was applied to determine the apoptosis of HT-29 cells.ELISA was performed to measure the levels of transforming growth factor β1 (TGF-β1).Western blotting was carried out to analyze the expression of p-Smad3, Smad4, Cyclin D1 and c-Myc protein.
      ResultsCCK8 results showed that the inhibitory effect of sulforaphane on the proliferation of HT-29 cells increased with the increase of drug concentration and time (P < 0.05 to P < 0.01).The apoptosis rate of HT-29 cells treated with 10, 20, 40 μmol/L sulforaphane was higher than that in control group (P < 0.05 to P < 0.01).The results of ELISA showed that the level of TGF-β1 in cell culture medium in 10, 20, 40 μmol/L sulforaphane group was lower than that in control group (P < 0.05 to P < 0.01).The expression of p-Smad3, Smad4, Cyclin D1 and c-Myc protein in HT-29 cells after treatment with 10, 20, 40 μmol/L sulforaphane was decreased compared with control group (P < 0.05 to P < 0.01).
      ConclusionsSulforaphane can inhibit the proliferation of colon cancer HT-29 cells, the mechanism of which might be related to the regulation of TGF-β1/Smad signaling pathway.

       

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