白术内酯Ⅰ通过调低CyclinD1/CDK4抑制胃癌细胞SGC-7901的增殖及机制

郭晨旭; 刘静波; 谢强; 许睿; 张明亮; 钱军

doi:10.13898/j.cnki.issn.1000-2200.2020.04.009

白术内酯Ⅰ通过调低CyclinD1/CDK4抑制胃癌细胞SGC-7901的增殖及机制

Atractylenolide Ⅰ inhibit the proliferation of gastric cancer cells SGC-7901 by lowering Cyclin D1/CDK4 and its mechanism

摘要

摘要:
目的探讨白术内酯I抑制胃癌细胞SGC-7901的增殖及可能机制。
方法 MTT法检测白术内酯Ⅰ对胃癌细胞SGC-7901增殖的抑制作用；流式细胞仪检测白术内酯Ⅰ作用后胃癌细胞SGC-7901的凋亡率及细胞周期的改变；Western blotting检测度白术内酯Ⅰ作用后胃癌细胞SGC-7901中Cyclin D1、CDK4蛋白的变化。
结果 MTT试验结果显示与对照组相比，白术内酯Ⅰ可抑制胃癌细胞SGC-7901的增殖，并且呈时间及剂量的依赖性（P < 0.05~P < 0.01）；流式细胞仪结果显示与对照组相比白术内酯Ⅰ能够明显促进胃癌细胞SGC-7901后的细胞凋亡，并且呈剂量的依赖性（P < 0.01），同时会增加细胞中G₁期细胞的比例（P < 0.01），减少G₂期细胞的比例（P < 0.01），并且呈剂量的依懒性（P < 0.05）；Western blotting结果显示同对照组相比白术内酯Ⅰ能够调低胃癌细胞SGC-7901中Cyclin D1、CDK4蛋白的表达（P < 0.01），并且呈剂量的依赖性（P < 0.05）。
结论白术内酯Ⅰ能通过调低Cyclin D1、CDK4蛋白进而改变细胞周期来抑制胃癌细胞SGC-7901的增殖同时促进其凋亡。

Abstract:
Objective To investigate the inhibition of atractylenolide Ⅰ on the proliferation of gastric cancer cell line SGC-7901 and its possible mechanism.
Methods After the atractylenolide Ⅰ treatment, the inhibitory effects of atractylenolide Ⅰ on the proliferation of gastric cancer cells SGC-7901 were detected using MTT assay, the apoptosis rate and cell cycle of gastric cancer cells SGC-7901 were detected using flow cytometry, and the changes of Cyclin D1 and CDK4 proteins in gastric cancer cells SGC-7901 were detected using Western blotting.
Results The results of MTT assay showed that atractylenolide Ⅰ could inhibit the proliferation of gastric cancer cells SGC-7901 in a timeand dose-dependent manner compared with the control group(P < 0.05 to P < 0.01).The results of flow cytometry showed that the atractylenolide Ⅰ could significantly promote the apoptosis of gastric cancer cells SGC-7901(P < 0.01), increase the ratio of G₁ phase cells(P < 0.01), and decrease the ratio of G₂ phase cells in a dose-dependent manner compared with the control group(P < 0.01).The results of Western blotting showed that atractylenolide Ⅰ could reduce the expression levels of Cyclin D1 and CDK4 proteins in SGC-7901 cells in a dose-dependent manner compared with the control group(P < 0.01).
Conclusions Atractylenolide Ⅰ can inhibit the proliferation, and promote apoptosis of gastric cancer cells SGC-7901 by reducing the levels of Cyclin D1 and CDK4 protein to change cell cycle.

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