LncRNA-ROR抑制结直肠癌细胞转移的作用研究

胡世丰; 雷春; 施炜; 查诚

doi:10.13898/j.cnki.issn.1000-2200.2020.05.003

LncRNA-ROR抑制结直肠癌细胞转移的作用研究

Study on the inhibition of LncRNA-ROR cells on colorectal cancer metastasis

摘要

摘要:
目的了解LncRNA-ROR在结直肠癌细胞转移中的作用。
方法在前期工作中，已经发现LncRNA-ROR在结直肠癌组织中低表达，并抑制肠癌细胞的增殖。进一步，我们采用Transwell功能研究LncRNA-ROR的过表达对结直肠癌细胞SW620迁移的影响；并采用划痕实验探索LncRNA-ROR过表达后结直肠癌细胞SW620伤痕愈合的变化。
结果SW620细胞在ROR过表达后，穿过人工膜的细胞数减少(P＜0.01)；在迁移和侵袭实验中，与对照组比较，SW620 LncRNA-ROR穿过Transwell膜的细胞数减少(P＜0.01)，侵袭能力减弱(P＜0.01)；划痕实验中，划痕48 h后LncRNA-ROR的细胞间间距大于对照组(P＜0.01)。
结论LncRNA-ROR抑制结直肠癌细胞的转移。

Abstract:
ObjectiveTo investigate the role of LncRNA-ROR cells in metastasis of colorectal cancer.
MethodsThe low expression of LncRNA-ROR in colorectal cancer was identified, which could inhibit the proliferation of colorectal cancer cells.The effects of the overexpression of LncRNA-ROR on colorectal cancer SW620 cells metastasis were explored using Transwell assay.The role of the overexpression of LncRNA-ROR on the healing of SW620 cells′ scratch was investigated using the wound healing assay.
ResultsAfter LncRNA-ROR was overexpressed in SW620 cells, the number of cells passing through the artificial membrane decreased(P＜0.01).In the migration and invasion experiments, compared with the control group, the number of cells with SW620 LncRNA-ROR passing the Transwell membrane decreased(P＜0.01), and the invasion ability decreased(P＜0.01).In the wound heding assay, the intercellular spacing of LncRNA-ROR after 48 h of scratching was greater than that in control group(P＜0.01).
ConclusionsLncRNA-ROR can inhibit the metastasis of colorectal cancer cells.

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